한빛사논문
- 조회404
Jinho Yoon, Hyun-Woong Kim, Minkyu Shin, Joungpyo Lim, Ji-Young Lee,
Sang-Nam Lee,* and Jeong-Woo Choi*
J. Yoon, H.-W. Kim, M. Shin, J. Lim, J.-Y. Lee, J.-W. Choi
Department of Chemical & Biomolecular Engineering Sogang University Seoul 04107, Republic of Korea
J. Yoon
Department of Chemistry and Chemical Biology Rutgers The State University of New Jersey 123 Bevier Road, Piscataway, NJ 08854, USA
S.-N. Lee
Uniance Gene Inc. Seoul 04107, Republic of Korea
J.Y. and H.-W.K. contributed equally to this work.
*Corresponding author.
Abstract
There have been several studies for demonstration of 2D neural network using living cells or organic/inorganic molecules, but to date, there is no report of development of a 3D neural network in vitro. Based on developed bionanohybrid composed of protein, DNA, molybdenum disulfide nanoparticles, and peptides for controlling electrophysiological states of living cells, here, the in vitro 3D neural network composed of the bionanohybrid, 3D neurospheroid and the microelectrode array (MEA) is developed. After production of the 3D neurospheroid derived from human neural stem cells, the bionanohybrid developed on the MEA successfully semi-penetrates the neurites of the 3D neurospheroid and forms the 3D neural network. The developed 3D neural network successfully exhibited the electrophysiological output signals of the 3D neurospheroid by transmitting the input signal applied by the bionanohybrid. Moreover, by using the selectively immobilized bionanohybrid on the MEA, the spatial input signal recognition in the neurospheroid of 3D neural network is realized for the first time. This newly developed in vitro 3D neural network provides a promising strategy to be applied in brain-on-a-chip, brain disease-related drug efficacy evaluation, bioelectronics, and bioelectronic medicine.
논문정보
- Research article
- 2022년 05월 (BRIC 등록일 2022-07-19)
- 국내(교신)+국외 연구진
- 바이오·의료융합 > 바이오센싱 및 나노바이오물질
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