한빛사논문
Yeo-Jun Yoon1,6, Donghyun Kim1,6, Kwon Yong Tak2, Seungyeon Hwang1, Jisun Kim1, Nam Suk Sim1, Jae-Min Cho1, Dojin Choi1, Yongmi Ji3, Junho K. Hur4, Hyunki Kim5, Jong-Eun Park2 & Jae-Yol Lim1,*
1Department of Otorhinolaryngology, Yonsei University College of Medicine, Seoul, South Korea. 2Graduate School of Medical Science and Engineering, Korean Advanced Institute of Science and Technology, Daejeon, South Korea. 3National Institute of Dental and Craniofacial Research, NIH, Bethesda, MD, USA. 4Department of Genetics, College of Medicine, Graduate School of Biomedical Science & Engineering, Hanyang University, Seoul, South Korea. 5Department of Pathology, Yonsei University College of Medicine, Seoul, South Korea. 6These authors contributed equally: Yeo-Jun Yoon, Donghyun Kim.
*Corresponding author.
Abstract
Salivary glands that produce and secrete saliva, which is essential for lubrication, digestion, immunity, and oral homeostasis, consist of diverse cells. The long-term maintenance of diverse salivary gland cells in organoids remains problematic. Here, we establish long-term murine and human salivary gland organoid cultures. Murine and human salivary gland organoids express gland-specific genes and proteins of acinar, myoepithelial, and duct cells, and exhibit gland functions when stimulated with neurotransmitters. Furthermore, human salivary gland organoids are established from isolated basal or luminal cells, retaining their characteristics. Single-cell RNA sequencing also indicates that human salivary gland organoids contain heterogeneous cell types and replicate glandular diversity. Our protocol also enables the generation of tumoroid cultures from benign and malignant salivary gland tumor types, in which tumor-specific gene signatures are well-conserved. In this study, we provide an experimental platform for the exploration of precision medicine in the era of tissue regeneration and anticancer treatment.
논문정보
관련 링크
연구자 키워드
관련분야 연구자보기
소속기관 논문보기
관련분야 논문보기
해당논문 저자보기