한빛사논문
- 조회615
Hosub Shin1,2*, Jeong Eun Park1*, Hye Rang Park1*, Woo Lee Choi1, Seung Hwa Yu3, Wonjun Koh1, Seungill Kim3,4, Hye Yeon Soh3, Nomar Espinosa Waminal1,5, Hadassah Roa Belandres5, Joo Young Lim1, Gibum Yi1,2, Jong Hwa Ahn1, June-Sik Kim1,6, Yong-Min Kim7, Namjin Koo7, Kyunghee Kim1, Sampath Perumal1, Taegu Kang1, Junghyo Kim3, Hosung Jang1,2, Dong Hyun Kang1, Ye Seul Kim1, Hyeon-Min Jeong3, Junwoo Yang1, Somin Song1, Suhyoung Park8, Jin A. Kim9, Yong Pyo Lim10, Beom-Seok Park11, Tzung-Fu Hsieh12, Tae-Jin Yang1,2,3,6, Doil Choi1,2,3,6, Hyun Hee Kim5, Soo-Seong Lee13 and Jin Hoe Huh1,2,3,6
1Department of Agriculture, Forestry and Bioresources, College of Agriculture and Life Science, Seoul National University, Seoul 08826, South Korea; 2Plant Genomics and Breeding Institute, Seoul National University, Seoul 08826, South Korea; 3Interdisciplinary Program in Agricultural Genomics, Seoul National University, Seoul 08826, South Korea; 4Department of Environmental Horticulture, University of Seoul, Seoul 02504, South Korea; 5Department of Life Science, Chromosome Research Institute, Sahmyook University, Seoul 01795, South Korea; 6Research Institute of Agriculture and Life Science, Seoul National University, Seoul 08826, South Korea; 7Korea Bioinformation Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, South Korea; 8Department of Horticultural Crop Research, National Institute of Horticultural and Herbal Science, Rural Development Administration, Wanju, Jeollabuk-do 55365, South Korea; 9Department of Agricultural Biotechnology, National Academy of Agricultural Science, Rural Development Administration, Jeonju, Jeollabuk-do 54874, South Korea; 10Department of Horticulture, Chungnam National University, Daejeon 34134, South Korea; 11Hongik Bio, Pyeongtaek, Gyeonggi-do 17977, South Korea; 12Plants for Human Health Institute, North Carolina State University, North Carolina Research Campus, Kannapolis, NC 27695, USA; 13BioBreeding Institute, Anseong, Gyeonggi-do 17544, South Korea
*These authors contributed equally to this work.
Author for correspondence: Jin Hoe Huh
Abstract
- Hybridization and polyploidization are pivotal to plant evolution. Genetic crosses between distantly related species are rare in nature due to reproductive barriers but how such hurdles can be overcome is largely unknown. Here we report the hybrid genome structure of xBrassicoraphanus, a synthetic allotetraploid of Brassica rapa and Raphanus sativus.
- We performed cytogenetic analysis and de novo genome assembly to examine chromosome behaviors and genome integrity in the hybrid. Transcriptome analysis was conducted to investigate expression of duplicated genes in conjunction with epigenome analysis to address whether genome admixture entails epigenetic reconfiguration.
- Allotetraploid xBrassicoraphanus retains both parental chromosomes without genome rearrangement. Meiotic synapsis formation and chromosome exchange are avoided between nonhomologous progenitor chromosomes. Reconfiguration of transcription network occurs, and less divergent cis-elements of duplicated genes are associated with convergent expression. Genome-wide DNA methylation asymmetry between progenitors is largely maintained but, notably, B. rapa-originated transposable elements are transcriptionally silenced in xBrassicoraphanus through gain of DNA methylation.
- Our results demonstrate that hybrid genome stabilization and transcription compatibility necessitate epigenome landscape adjustment and rewiring of cis–trans interactions. Overall, this study suggests that a certain extent of genome divergence facilitates hybridization across species, which may explain the great diversification and expansion of angiosperms during evolution.
논문정보
- Research article
- 2022년 04월 (BRIC 등록일 2022-06-24)
- 국내(교신)+국외 연구진
- 생명과학 > 생화학
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