한빛사논문
- 조회611
Sunjoo Joo1,4, Thamali Kariyawasam1,4, Minjae Kim2, EonSeon Jin2, Ursula Goodenough3 & Jae-Hyeok Lee1,*
1Department of Botany, University of British Columbia, 6270 University Blvd, Vancouver, BC V6T 1Z4, Canada. 2Department of Life Sciences, Hanyang University, Seoul 133-791, Republic of Korea. 3Department of Biology, Washington University in St. Louis, 1 Brookings Dr, St. Louis, MO 63110, USA. 4These authors contributed equally: Sunjoo Joo, Thamali Kariyawasam.
*Corresponding author.
Abstract
Most sexual organisms inherit organelles from one parent, commonly by excluding organelles from the smaller gametes. However, post-mating elimination of organelles derived from one gamete ensures uniparental inheritance, where the underlying mechanisms to distinguish organelles by their origin remain obscure. Mating in Chlamydomonas reinhardtii combines isomorphic plus and minus gametes, but chloroplast DNA from minus gametes is selectively degraded in zygotes. Here, we identify OTU2p (otubain protein 2), encoded in the plus mating-type locus MT+, as the protector of plus chloroplast. Otu2p is an otubain-like deubiquitinase, which prevents proteasome-mediated degradation of the preprotein translocase of the outer chloroplast membrane (TOC) during gametogenesis. Using OTU2p-knockouts and proteasome inhibitor treatment, we successfully redirect selective DNA degradation in chloroplasts with reduced TOC levels regardless of mating type, demonstrating that plus-specific Otu2p establishes uniparental chloroplast DNA inheritance. Our work documents that a sex-linked organelle quality control mechanism drives the uniparental organelle inheritance without dimorphic gametes.
논문정보
- Research article
- 2022년 03월 (BRIC 등록일 2022-05-31)
- 국외 연구진
- 생명과학 > 발생생물학
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