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한빛사논문

조회435

University of Illinois at Urbana-Champaign, UNIST

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J. Am. Chem. Soc., Published online 18 March 2022 | https://doi.org/10.1021/jacs.1c11543 Noninvasive and Spatiotemporal Control of DNAzyme-Based Imaging of Metal Ions In Vivo Using High-Intensity Focused Ultrasound

Xiaojing Wang ^{†, ‡, ◆}, Gun Kim ^{∥, ⊥, ∇ ◆}, James L. Chu ^{∥, ⊥, ◆}, Tingjie Song ^†, Zhenglin Yang ^{‡, §}, Weijie Guo ^{‡, §}, Xiangli Shao ^†, Michael L. Oelze ^{∥, ⊥, ○}, King C. Li ^{∥, ⊥, #, *}, and Yi Lu ^{†, ‡, §, *}

^† Department of Chemistry, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, United States.
^‡ Department of Biochemistry, University of Illinois at Urbana−Champaign, Urbana, Illinois 61801, United States.
^§ Department of Chemistry, University of Texas at Austin, Austin, TX 78712, United States
^∥Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, United States.
^⊥Carle Illinois College of Medicine, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, United States.
^# Department of Bioengineering, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, United States.
^∇ Department of Urban and Environmental Engineering, Ulsan National Institute of Science and Technology (UNIST), Ulsan 44919, Republic of Korea
^○Department of Electrical and Computer Engineering, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, United States.

^◆X.W., G.K., and J.L.C contributed equally to this work.
^*Corresponding Author

Abstract

Detecting metal ions invivo with a high spatiotemporal resolution is critical to understanding the roles of the metal ions in both healthy and disease states. Although spatiotemporal controls of metal-ion sensors using light have been demonstrated, the lack of penetration depth in tissue and in vivo has limited their application. To overcome this limitation, we herein report the use of high-intensity focused ultrasound (HIFU) to remotely deliver on-demand, spatiotemporally resolved thermal energy to activate the DNAzyme sensors at the targeted region both in vitro and in vivo. A Zn²⁺-selective DNAzyme probe is inactivated by a protector strand to block the formation of catalytic enzyme structure, which can then be activated by an HIFU-induced increase in the local temperature. With this design, Zn²⁺-specific fluorescent resonance energy transfer (FRET) imaging has been demonstrated by the new DNAzyme-HIFU probes in both HeLa cells and mice. The current method can be applied to monitor many other metal ions for in vivo imaging and medical diagnosis using metal-specific DNAzymes that have either been obtained or can be selected using in vitro selection.

논문정보

형식Research article
게재일2022년 03월 (BRIC 등록일 2022-05-25)
연구진국내+국외 연구진
분야 바이오·의료융합 > 바이오센싱 및 나노바이오물질

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