여강인 (연세대학교)

한빛사논문

조회373

연세대학교

CV File 9 kb

Biosens. Bioelectron., Available online 14 April 2022, Volume 210 | https://doi.org/10.1016/j.bios.2022.114235 Ultra-sensitive dielectrophoretic surface charge multiplex detection inside a micro-dielectrophoretic device

Kang In Yeo ^a,1, Insu Park ^b,1, Sang Hyun Lee ^a, Sei Young Lee ^a, Woo-Jin Chang ^c, Rashid Bashir ^b,d,e,f, Seungyeop Choi ^a,*, Sang Woo Lee ^a,*

^a Department of Biomedical Engineering, Yonsei University, Wonju, 26493, Republic of Korea ^b Holonyak Micro and Nanotechnology Laboratory, University of Illinois at Urbana—Champaign, Urbana, IL, 61801, USA ^c Mechanical Engineering Department, University of Wisconsin-Milwaukee, Milwaukee, WI, 53211, USA ^d Department of Mechanical Science and Engineering, University of Illinois at Urbana—Champaign, Urbana, IL, 61801, USA ^e Department of Bioengineering, University of Illinois at Urbana—Champaign, Urbana, IL, 61801, USA ^f Materials Research Laboratory, University of Illinois at Urbana—Champaign, Urbana, IL, 61801, USA

^* Corresponding author.
¹ These authors contributed equally to this work.

Abstract

Label-free dielectrophoretic force-based surface charge detection has shown great potential for highly sensitive and selective sensing of metal ions and small biomolecules. However, this method suffers from a complex calibration process and measurement signal interference in simultaneous multi-analyte detection, thus creating difficulties in multiplex detection. We have developed a method to overcome these issues based on the optical discrimination of the dielectrophoretic behaviors of multiple microparticle probes considering the surface charge difference before and after self-assembling conjugation. In this report, we demonstrate and characterize this dielectrophoretic force-based surface charge detection method with particle probes functionalized by various biomolecules. This technique achieved an attomolar limit of detection (LOD) for Hg²⁺ in distilled water and a femtomolar LOD in drinking water using DNA aptamer-functionalized particle probes. More importantly, using two different DNA aptamer-functionalized particle probes for Hg²⁺ and Ag⁺, label-free dielectrophoretic multiplex detection of these species in drinking water with a femtomolar and a nanomolar LOD was achieved for the first time.

논문정보

형식Research article
게재일2022년 04월 (BRIC 등록일 2022-05-27)
연구진국내(교신)+국외 연구진
분야 바이오·의료융합 > 바이오센싱 및 나노바이오물질

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