한빛사논문
Jaeyoung K. Jung 1,2,3, Chloé M. Archuleta1,2,3, Khalid K. Alam 4 and Julius B. Lucks 1,2,3,4,5,*
1Department of Chemical and Biological Engineering, Northwestern University, Evanston, IL, USA. 2Center for Synthetic Biology, Northwestern University, Evanston, IL, USA. 3Center for Water Research, Northwestern University, Evanston, IL, USA. 4Stemloop, Inc., Evanston, IL, USA. 5Interdisciplinary Biological Sciences Graduate Program, Northwestern University, Evanston, IL, USA.
*Corresponding author.
Abstract
Cell-free biosensors are powerful platforms for monitoring human and environmental health. Here, we expand their capabilities by interfacing them with toehold-mediated strand displacement circuits, a dynamic DNA nanotechnology that enables molecular computation through programmable interactions between nucleic acid strands. We develop design rules for interfacing a small molecule sensing platform called ROSALIND with toehold-mediated strand displacement to construct hybrid RNA–DNA circuits that allow fine-tuning of reaction kinetics. We use these design rules to build 12 different circuits that implement a range of logic functions (NOT, OR, AND, IMPLY, NOR, NIMPLY, NAND). Finally, we demonstrate a circuit that acts like an analog-to-digital converter to create a series of binary outputs that encode the concentration range of the molecule being detected. We believe this work establishes a pathway to create ‘smart’ diagnostics that use molecular computations to enhance the speed and utility of biosensors.
논문정보
관련 링크
관련분야 연구자보기
소속기관 논문보기
관련분야 논문보기
해당논문 저자보기