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Biosens. Bioelectron., Volume 197, 1 February 2022, 113753 | https://doi.org/10.1016/j.bios.2021.113753 Microfluidic device for one-step detection of breast cancer-derived exosomal mRNA in blood using signal-amplifiable 3D nanostructure

Jaewoo Lim^a,b, Byunghoon Kang^a, Hye Young Son^c,d, Byeonggeol Mun^e, Yong-Min Huh^c,d,f, Hyun Wook Rho^c, Taejoon Kang^a, Jeong Moon^a,g, Jae-Jong Lee^h, Seung Beom Seo^a,i, Soojin Jang^a,b, Seong Uk Son^a,b, Juyeon Jung^a,b, Seungjoo Haam^e,**, Eun-Kyung Lim^a,b,*

^aBionanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahak-ro, Yuseong-gu, Daejeon, 34141, Republic of Korea
^bDepartment of Nanobiotechnology, KRIBB School of Biotechnology, University of Science and Technology, 125 Gwahak-ro, Yuseong-gu, Daejeon, 34113, Republic of Korea
^cDepartment of Radiology, College of Medicine, Yonsei University, 50-1 Yonsei-ro, Seodaemun-gu, Seoul, 03772, Republic of Korea ^dSeverance Biomedical Science Institute, College of Medicine, Yonsei University, 50-1 Yonsei-ro, Seodaemun-gu, Seoul, 03722, Republic of Korea ^eDepartment of Chemical & Biomolecular Engineering, College of Engineering, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul, 03722, Republic of Korea ^fYUHS-KRIBB Medical Convergence Research Institute, 50-1 Yonsei-ro, Seodaemun-gu, Seoul, 03722, Republic of Korea ^gDepartment of Chemical and Biomolecular Engineering (BK 21+ Program), KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon, 34141, Republic of Korea ^hDepartment of Nano Manufacturing Technology, Korea Institute of Machinery and Materials (KIMM), 156 Gajeongbuk-ro, Yuseong-gu, Daejeon, 34103, Republic of Korea ⁱDepartment of Cogno-Mechatronics Engineering, Pusan National University, 2 Busandaehak-ro 63beon-gil, Geumjeong-gu, Busan 46241, Republic of Korea

^*Corresponding author.
^**Corresponding author

Abstract

Metastasis attributed to approximately 90% of cancer-related deaths; hence, the detection of metastatic tumor–derived components in the blood assists in determining cancer recurrence and patient survival. Microfluidic–based sensors facilitate analysis of small fluid volumes and represent an accurate, rapid, and user-friendly method of field diagnoses. In this study, we have developed a microfluidic chip-based exosomal mRNA sensor (exoNA-sensing chip) for the one-step detection of exosomal ERBB2 in the blood by integrating a microfluidic chip and 3D-nanostructured hydrogels. The exoNA-sensing chip is a vacuum-driven power-free microfluidic chip that can accurately control the flow of trace fluids (<100 μL). The sensing part of the exoNA-sensing chip includes 3D-nanostructured hydrogels capable of detecting ERBB2 and a reference gene by amplifying a fluorescent signal via an enzyme-free catalytic hairpin assembly reaction at room temperature. This hydrogel offers a detection limit of 58.3 fM with good selectivity for target sequences. The performance of the exoNA-sensing chip was evaluated by testing in vitro and in vivo samples and was proven to be effective for cancer diagnosis and liquid biopsies.

논문정보

형식Research article
게재일2022년 02월 (BRIC 등록일 2022-04-08)
연구진국내 연구진
분야 바이오·의료융합 > 바이오센싱 및 나노바이오물질

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