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Chem. Eng. J., Volume 442, Part 1, 15 August 2022, 136143 | https://doi.org/10.1016/j.cej.2022.136143 FRET-based hACE2 receptor mimic peptide conjugated nanoprobe for simple detection of SARS-CoV-2

Byunghoon Kang^a, Youngjin Lee^b, Jaewoo Lim^a,c, Dongeun Yong^d, Young Ki Choi^e,f, Sun Woo Yoon^a, Seungbeom Seo^a,g, Soojin Jang^a,c, Seong Uk Son^a,c, Taejoon Kang^a, Juyeon Jung^a,c, Kyu-Sun Lee^a, Myung Hee Kim^b,*, Eun-Kyung Lim^a,c,*

^aBioNanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea ^bMetabolic Regulation Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea ^cDepartment of Nanobiotechnology, KRIBB School of Biotechnology, University of Science and Technology (UST), 217 Gajeong-ro, Yuseong-gu, Daejeon 34113, Republic of Korea ^dDepartment of Laboratory Medicine and Research Institute of Bacterial Resistance, College of Medicine, Yonsei University, 50-1 Yonsei-ro, Seodaemun-gu, Seoul 03722, Republic of Korea ^eDepartment of Microbiology, College of Medicine and Medical Research Institute, Chungbuk National University, 776 1sunhwan-ro, Seowon-gu, Cheongju 28644, Republic of Korea ^fCenter for Study of Emerging and Re-emerging Viruses, Korea Virus Research Institute, Institute for Basic Science (IBS), Daejeon 34126, Republic of Korea ^gDepartment of Cogno-Mechatronics Engineering, Pusan National University, 2 Busandaehak-ro, Gumjeong-gu, Busan 46241, Republic of Korea

^*Corresponding author.

Abstract

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection has led to a pandemic of acute respiratory disease, namely coronavirus disease (COVID-19). This disease threatens human health and public safety. Early diagnosis, isolation, and prevention are important to suppress the outbreak of COVID 19 given the lack of specific antiviral drugs to treat this disease and the emergence of various variants of the virus that cause breakthrough infections even after vaccine administration. Simple and prompt testing is paramount to preventing further spread of the virus. However, current testing methods, namely RT-PCR, is time-consuming. Binding of the SARS-CoV-2 spike (S) glycoprotein to human angiotensin-converting enzyme 2 (hACE2) receptor plays a pivotal role in host cell entry. In the present study, we developed a hACE2 mimic peptide beacon (COVID19-PEB) for simple detection of SARS-CoV-2 using a fluorescence resonance energy transfer system. COVID19-PEB exhibits minimal fluorescence in its ‘‘closed’’ hairpin structure; however, in the presence of SARS-CoV-2, the specific recognition of the S protein receptor-binding domain by COVID19-PEB causes the beacon to assume an ‘‘open’’ structure that emits strong fluorescence. COVID19-PEB can detect SARS-CoV-2 within 3 h or even 50 min and exhibits strong fluorescence even at low viral concentrations, with a detection limit of 4 × 10³ plaque-forming unit/test. Furthermore, in SARS-CoV-2-infected patient samples confirmed using polymerase chain reaction, COVID19-PEB accurately detected the virus. COVID19-PEB could be developed as a rapid and accurate diagnostic tool for COVID-19.

논문정보

형식Research article
게재일2022년 08월 (BRIC 등록일 2022-04-08)
연구진국내 연구진
분야 바이오·의료융합 > 바이오센싱 및 나노바이오물질

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