한빛사논문
- 조회433
Daeho Sunga,b,1, Chan Lima,b,1, Masatoshi Takagic, Chulho Junga,b, Heemin Leea,b, Do Hyung Choa,b, Jae-Yong Shina,b, Kangwoo Ahnd, Junha Hwanga,b, Daewoong Namb,e, Yoshiki Kohmuraf, Tetsuya Ishikawaf, Do Young Nohd,g, Naoko Imamotoc, Jae-Hyung Jeona,b,h,2, and Changyong Songa,b,h,2
aDepartment of Physics, Pohang University of Science and Technology, Pohang 37673, Korea; bPhoton Science Center, Pohang University of Science and Technology, Pohang 37673, Korea; cCellular Dynamics Laboratory, RIKEN, Saitama 351-0198, Japan; dDepartment of Physics and Photon Science, Gwangju Institute of Science and Technology, Gwangju 61005, Korea; ePohang Accelerator Laboratory, Pohang 37673, Korea; fRIKEN SPring-8 Center, Hyogo 679-5148, Japan; gInstitute for Basic Science, Daejeon 34126, Korea; and hAsia Pacific Center for Theoretical Physics, Pohang University of Science and Technology, Pohang 37673, Korea
1D.S. and C.L. contributed equally to this work.
2To whom correspondence may be addressed.
Abstract
DNA molecules are atomic-scale information storage molecules that promote reliable information transfer via fault-free repetitions of replications and transcriptions. Remarkable accuracy of compacting a few-meters-long DNA into a micrometer-scale object, and the reverse, makes the chromosome one of the most intriguing structures from both physical and biological viewpoints. However, its three-dimensional (3D) structure remains elusive with challenges in observing native structures of specimens at tens-of-nanometers resolution. Here, using cryogenic coherent X-ray diffraction imaging, we succeeded in obtaining nanoscale 3D structures of metaphase chromosomes that exhibited a random distribution of electron density without characteristics of high-order folding structures. Scaling analysis of the chromosomes, compared with a model structure having the same density profile as the experimental results, has discovered the fractal nature of density distributions. Quantitative 3D density maps, corroborated by molecular dynamics simulations, reveal that internal structures of chromosomes conform to diffusion-limited aggregation behavior, which indicates that 3D chromatin packing occurs via stochastic processes.
논문정보
- Research article
- 2021년 11월 (BRIC 등록일 2022-04-01)
- 국내(교신)+국외 연구진
- 생명과학 > 세포생물학
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