한빛사논문
- 조회765
Choon-Tak Kwon 1,2, Lingli Tang3,4, Xingang Wang 1, Iacopo Gentile1, Anat Hendelman 1, Gina Robitaille 1,5, Joyce Van Eck 6,7, Cao Xu 3,4,* and Zachary B. Lippman 1,5,*
1School of Biological Sciences, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, NY, USA. 2Department of Horticultural Biotechnology, Kyung Hee University, Yongin, Republic of Korea. 3State Key Laboratory of Plant Genomics, National Center for Plant Gene Research, CAS-JIC Centre of Excellence for Plant and Microbial Science (CEPAMS), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China. 4University of Chinese Academy of Sciences, Beijing, China. 5Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, NY, USA. 6Boyce Thompson Institute, Ithaca, NY, USA. 7Plant Breeding and Genetics Section, School of Integrative Plant Science, Cornell University, Ithaca, NY, USA.
*Corresponding author.
Abstract
Gene duplications are a hallmark of plant genome evolution and a foundation for genetic interactions that shape phenotypic diversity1,2,3,4,5. Compensation is a major form of paralogue interaction6,7,8 but how compensation relationships change as allelic variation accumulates is unknown. Here we leveraged genomics and genome editing across the Solanaceae family to capture the evolution of compensating paralogues. Mutations in the stem cell regulator CLV3 cause floral organs to overproliferate in many plants9,10,11. In tomato, this phenotype is partially suppressed by transcriptional upregulation of a closely related paralogue12. Tobacco lost this paralogue, resulting in no compensation and extreme clv3 phenotypes. Strikingly, the paralogues of petunia and groundcherry nearly completely suppress clv3, indicating a potent ancestral state of compensation. Cross-species transgenic complementation analyses show that this potent compensation partially degenerated in tomato due to a single amino acid change in the paralogue and cis-regulatory variation that limits its transcriptional upregulation. Our findings show how genetic interactions are remodelled following duplications and suggest that dynamic paralogue evolution is widespread over short time scales and impacts phenotypic variation from natural and engineered mutations.
논문정보
- Research article
- 2022년 03월 (BRIC 등록일 2022-03-30)
- 국내+국외 연구진
- 생명과학 > 식량작물 및 원예작물
댓글 작성 로그인
팝업 닫기관련 링크
연구자 키워드
관련분야 연구자보기
소속기관 논문보기
관련분야 논문보기
해당논문 저자보기
