한빛사논문
- 조회903
Letao Yang, Brian M. Conley, Christopher Rathnam, Hyeon-Yeol Cho, Thanapat Pongkulapa, Brandon Conklin, and Ki-Bum Lee*
Department of Chemistry and Chemical Biology Rutgers University, the State University of New Jersey Piscataway, NJ 08854, U.S.A.
*Corresponding author.
Abstract
Biophysical cues, such as nanotopographies of extracellular matrix (ECM), are key cell regulators for direct cell reprogramming. Therefore, high-throughput methods capable of systematically screening a wide range of biophysical cue-regulated cell reprogramming are increasingly needed for tissue engineering and regenerative medicine. Here, we report the development of a dynamic laser interference lithography (DIL) to generate large-scale combinatorial biophysical cue (CBC) arrays with diverse micro/nanostructures at higher complexities than most current arrays. Using CBC arrays, a high-throughput cell mapping method is further demonstrated for the systematic investigation of biophysical cue-mediated direct cell reprogramming. This CBC array-based high-throughput cell screening approach facilitates the rapid identification of unconventional hierarchical nanopatterns that induce the direct reprogramming of human fibroblasts into neurons through epigenetic modulation mechanisms. In this way, we successfully demonstrate DIL for generating highly complex CBC arrays and establish CBC array-based cell screening as a valuable strategy for systematically investigating the role of biophysical cues in cell reprogramming.
논문정보
- Research article
- 2022년 03월 (BRIC 등록일 2022-03-22)
- 국외 연구진
- 바이오·의료융합 > 바이오센싱 및 나노바이오물질
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