김재범 (서울대학교)

한빛사논문

조회773

서울대학교

CV File 482 kb

Cell Metab., Available online 11 January 2022 | https://doi.org/10.1016/j.cmet.2021.11.014 Distinct properties of adipose stem cell subpopulations determine fat depot-specific characteristics

Hahn Nahmgoong^1,5, Yong Geun Jeon^1,5, Eun Seo Park^2,5, Yoon Ha Choi², Sang Mun Han¹, Jeu Park¹, Yul Ji¹, Jee Hyung Sohn¹, Ji Seul Han¹, Ye Young Kim¹, Injae Hwang¹, Yun Kyung Lee³, Jin Young Huh¹, Sung Sik Choe¹, Tae Jung Oh³, Sung Hee Choi³, Jong Kyoung Kim^2,4,*, Jae Bum Kim^1,6,*

¹National Creative Research Initiatives Center for Adipocyte Structure and Function, Institute of Molecular Biology and Genetics, School of Biological Sciences, Seoul National University, Seoul 08826, Republic of Korea
²Department of New Biology, DGIST, Daegu 42988, Republic of Korea
³Internal Medicine, Seoul National University College of Medicine & Seoul National University Bundang Hospital, Seoul 03080, Republic of Korea
⁴Present address: Department of Life Sciences, Pohang University of Science and Technology, Pohang 37673, Republic of Korea
⁵These authors contributed equally
⁶Lead contact

^*Corresponding author

Abstract

In mammals, white adipose tissues are largely divided into visceral epididymal adipose tissue (EAT) and subcutaneous inguinal adipose tissue (IAT) with distinct metabolic properties. Although emerging evidence suggests that subpopulations of adipose stem cells (ASCs) would be important to explain fat depot differences, ASCs of two fat depots have not been comparatively investigated. Here, we characterized heterogeneous ASCs and examined the effects of intrinsic and tissue micro-environmental factors on distinct ASC features. We demonstrated that ASC subpopulations in EAT and IAT exhibited different molecular features with three adipogenic stages. ASC transplantation experiments revealed that intrinsic ASC features primarily determined their adipogenic potential. Upon obesogenic stimuli, EAT-specific SDC1+ ASCs promoted fibrotic remodeling, whereas IAT-specific CXCL14+ ASCs suppressed macrophage infiltration. Moreover, IAT-specific BST2high ASCs exhibited a high potential to become beige adipocytes. Collectively, our data broaden the understanding of ASCs with new insights into the origin of white fat depot differences.

Keywords : single-cell RNA-seq, adipose stem cells, fat depots, adipose tissue remodeling, obesity, adipogenesis, inflammation, fibrosis, beige adipocytes, lymph nodes

논문정보

형식Research article
게재일2022년 01월 (BRIC 등록일 2022-02-24)
연구진국내 연구진
분야 생명과학 > 분자생물학

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