한빛사논문
- 조회550
Jaehi Kim1,†, Do Won Hwang2,3,†, Heung Su Jung4,†, Kyu Wan Kim2, Xuan‑Hung Pham1, Sang‑Hun Lee5, Jung Woo Byun2, Wooyeon Kim1, Hyung‑Mo Kim1,8, Eunil Hahm1, Kyeong‑min Ham1, Won‑Yeop Rho6, Dong Soo Lee2,7,* and Bong‑Hyun Jun1,*
1Department of Bioscience and Biotechnology, Konkuk University, Seoul, Republic of Korea. 2Department of Nuclear Medicine, Seoul National University College of Medicine, Seoul, Republic of Korea. 3THERABEST, Co. Inc., Seocho‑daero 40‑gil, Seoul, Republic of Korea. 4Company of Global Zeus, Hwaseong, Gyeonggi‑do, Republic of Korea. 5Department of Chemical and Biological Engineering, Hanbat University, Daejeon, Republic of Korea. 6School of International Engineering and Science, Jeonbuk National University, Jeonju‑si, Jeollabuk‑do, Republic of Korea. 7Department of Molecular Medicine and Biopharmaceutical Sciences, Graduate School of Convergence Science and Technology and College of Medicine or College of Pharmacy, Seoul National University, Seoul, Republic of Korea. 8KIURI Research Center, Ajou University, Suwon, Republic of Korea.
†Jaehi Kim, Do Won Hwang and Heung Su Jung contributed equally to this work
*Correspondence to Dong Soo Lee or Bong-Hyun Jun.
Abstract
Background
Quantum dots (QDs) have been used as fluorophores in various imaging fields owing to their strong fluorescent intensity, high quantum yield (QY), and narrow emission bandwidth. However, the application of QDs to bio-imaging is limited because the QY of QDs decreases substantially during the surface modification step for bio-application.
Results
In this study, we fabricated alloy-typed core/shell CdSeZnS/ZnS quantum dots (alloy QDs) that showed higher quantum yield and stability during the surface modification for hydrophilization compared with conventional CdSe/CdS/ZnS multilayer quantum dots (MQDs). The structure of the alloy QDs was confirmed using time-of-flight medium-energy ion scattering spectroscopy. The alloy QDs exhibited strong fluorescence and a high QY of 98.0%. After hydrophilic surface modification, the alloy QDs exhibited a QY of 84.7%, which is 1.5 times higher than that of MQDs. The QY was 77.8% after the alloy QDs were conjugated with folic acid (FA). Alloy QDs and MQDs, after conjugation with FA, were successfully used for targeting human KB cells. The alloy QDs exhibited a stronger fluorescence signal than MQD; these signals were retained in the popliteal lymph node area for 24 h.
Conclusion
The alloy QDs maintained a higher QY in hydrophilization for biological applications than MQDs. And also, alloy QDs showed the potential as nanoprobes for highly sensitive bioimaging analysis.
논문정보
- Research article
- 2022년 01월 (BRIC 등록일 2022-02-15)
- 국내 연구진
- 바이오·의료융합 > 바이오센싱 및 나노바이오물질
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