한빛사논문
- 조회488
POSTECH
Jaeseung Youn1,6, Hyeonjun Hong1,6, Woojung Shin2, Dohui Kim1, Hyun Jung Kim3,∗ and Dong Sung Kim1,4,5,∗
1Department of Mechanical Engineering, Pohang University of Science and Technology (POSTECH), 77 Cheongam-ro, Pohang, Gyeongbuk 37673, Republic of Korea
2Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA 02115, United States of America 3Department of Biomedical Engineering, The University of Texas at Austin, 107 W. Dean Keeton St., Austin, TX 78712, United States of America 4Department of Chemical Engineering, Pohang University of Science and Technology (POSTECH), 77, Cheongam-ro, Nam-gu, Pohang, Gyeongbuk 37673, Republic of Korea 5Institute for Convergence Research and Education in Advanced Technology, Yonsei University, 50, Yonsei-ro, Seodaemun-gu, Seoul 03722, Republic of Korea
6These authors contributed equally to this work.
∗Authors to whom any correspondence should be addressed.
Abstract
An extracellular matrix (ECM) membrane made up of ECM hydrogels has great potentials to develop a physiologically relevant organ-on-a-chip because of its biochemical and biophysical similarity to in vivo basement membranes (BMs). However, the limited mechanical stability of the ECM hydrogels makes it difficult to utilize the ECM membrane in long-term and dynamic cell/tissue cultures. This study proposes a thin but robust and transparent ECM membrane reinforced with silk fibroin (SF)/polycaprolactone (PCL) nanofibers, which is achieved by in situ self-assembly throughout a freestanding SF/PCL nanofiber scaffold. The SF/PCL nanofiber-reinforced ECM (NaRE) membrane shows biophysical characteristics reminiscent of native BMs, including small thickness (<5 μm), high permeability (<9 × 10−5 cm s−1), and nanofibrillar architecture (∼10–100 nm). With the BM-like characteristics, the nanofiber reinforcement ensured that the NaRE membrane stably supported the construction of various types of in vitro barrier models, from epithelial or endothelial barrier models to complex co-culture models, even over two weeks of cell culture periods. Furthermore, the stretchability of the NaRE membrane allowed emulating the native organ-like cyclic stretching motions (10%–15%) and was demonstrated to manipulate the cell and tissue-level functions of the in vitro barrier model.
논문정보
- Research article
- 2022년 02월 (BRIC 등록일 2022-02-16)
- 국내(교신)+국외 연구진
- 바이오·의료융합 > 바이오소재
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