한빛사논문
Kyung Ho Kima,1, Chul Soon Parka,b,1, Seon Joo Parka, Jinyeong Kima, Sung Eun Seoa, Jai Eun Ana, Siyoung Haa, Joonwon Baec, Sooyeol Phyod,e, Jiwon Leed,f, Kayoung Kimg, Dongseok Moonh, Tai Hyun Parkh, Hyun Seok Songg,*, Oh Seok Kwona,i,*
aInfectious Disease Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, 34141, Republic of Korea
bDrug Manufacturing Center, Daegu-Gyeongbuk Medical Innovation Foundation(DGMIF), Daegu 41061, Republic of Korea
cDepartment of Applied Chemistry, Dongduk Women’s University, Seoul, 02748, Republic of Korea
dCenter for Environment, Health and Welfare Research, Korea Institute of Science and Technology, Seoul, 02792, Republic of Korea
eDepartment of Materials Science and Engineering, Korea University, Seoul, Republic of Korea
fDivision of Energy & Environment Technology, Korea University of Science and Technology(UST), Republic of Korea
gSensor System Research Center, Korea Institute of Science and Technology, Seoul, 02792, Republic of Korea
hSchool of Chemical and Biological Engineering, Institute of Chemical Processes, Seoul National University, Seoul, 08826, Republic of Korea
iDepartment of Nanobiotechnology, Korea University of Science and Technology (UST), Republic of Korea
1These authors contributed equally to this work.
*Corresponding author
Abstract
Monitoring food spoilage is one of the most effective methods for preventing food poisoning caused by biogenic amines or microbes. Therefore, various analytical techniques have been introduced to detect low concentrations of cadaverine (CV) and putrescine (PT), which are representative biogenic polyamines involved in food spoilage (5–8 ppm at the stage of initial decomposition after storage for 5 days at 5 °C and 17–186 ppm at the stage of advanced decomposition after storage for 7 days at 5 °C). Although previous methods showed selective CV and PT detection even at low concentrations, the use of these methods remains challenging in research areas that require in-situ, real-time, on-site monitoring. In this study, we demonstrated for the first time an in-situ high-performance chemical receptor-conjugated graphene electronic nose (CRGE-nose) whose limits of detection (LODs), 27.04 and 7.29 ppb, for CV and PT are up to 102 times more sensitive than those of conventional biogenic amine sensors. Specifically, the novel chemical receptors 2,7-bis(3-morpholinopropyl)benzo[lmn][3,8] phenanthroline-1,3,6,8(2H,7H)-tetraone (NaPhdiMor (NPM)) and 2,7-bis(2-((3-morpholinopropyl)amino)ethyl)benzo[lmn][3,8]phenanthroline-1,3,6,8(2H,7H)-tetraone (NaPhdiEtAmMor (NPEAM)) were designed on the basis of density functional theory (DFT) calculations, and their interaction mechanism was characterized by a DFT 3D simulation. Interestingly, the CRGE-nose was connected on a micro sim chip substrate via wire bonding and then integrated into wireless portable devices, resulting in a cost-effective, high-performance prototype CRGE-nose device capable of on-site detection. The portable CRGE-nose can be used for in-situ monitoring of CV and PT concentration changes as low as 27.04 and 7.29 ppb in real meats such as pork, beef, lamb and chicken.
Keywords : Chemical receptor, Graphene, Cadaverine, Putrescine, Gas sensor, Real-time monitoring, Portable biosensors
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