한빛사논문
- 조회942
Jae Won Song1,2, Jungyo Suh3,4, Seok Won Lee2, Jung Ki Yoo2,5, Uijeong Lee6, Jang Hee Han7, Cheol Kwak3, Minyong Kang8,9, Yi Rang Kim6, Chang Wook Jeong3,* Jin Woo Choi1,*
1Department of Pharmacy and Department of Regulatory Science, College of Pharmacy, Kyung Hee University, Seoul, 02447, Republic of Korea.
2Department of Biomedical and Pharmaceutical Sciences, Kyung Hee University, Seoul, 02447, Republic of Korea.
3Department of Urology, Seoul National University Hospital, Seoul National University College of Medicine, Seoul, 03080, Republic of Korea.
4Department of Urology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, 05505, Republic of Korea.
5Research Center of Curigin Ltd., Seoul, 04778, Republic of Korea.
6Artificial Intelligence Laboratory of Oncocross Ltd., Seoul, 04168, Republic of Korea.
7Department of Urology, Seoul National University Hospital, Seoul, 03080, Republic of Korea.
8Department of Urology, Samsung Medical Center, Sungkyunkwan University School of Medicine, Department of Health Sciences and Technology SAIHST Sungkyunkwan University.
9Samsung Genome Institute, Samsung Medical Center, Seoul, 06351, Republic of Korea.
J.W.S. and J.S. have contributed equally to this work.
*Corresponding author.
Abstract
As epithelial cells in the circulation are considered to originate from the tumor, the epithelial cell adhesion molecule has been commonly used as a standard marker for circulating tumor cells (CTCs) isolation. However, it seems to disappear after the epithelial–mesenchymal transition that most cancer cells undergo for intravasation. Thus, more advanced techniques for CTC detection are needed to better understand the clinical significance of CTCs. A cancer cell-specifically-infecting or replicating virus that codes a fluorescent monitor gene can be a solution to efficiently detect CTCs. Thus, the authors designed an adenovirus to bind to desmoglein-2, which is highly expressed in most cancer cells. A cancer-specific human telomerase reverse transcriptase promoter is inserted to control a viral E1 region. The adenovirus is utilized to compare the number of CTCs from renal cell carcinoma and prostate cancer patients before and after surgery. The isolated two or three CTCs are eligible for whole genome sequencing. The genomic analysis proves the difference of variants between primary tumors and CTCs. Taken together, it is a fast and exact serial method for CTC isolation and the enriched genome sequencing may be used to determine the prognosis and as a point-of-care system for patients with cancer.
논문정보
- Research article
- 2022년 01월 (BRIC 등록일 2022-02-08)
- 국내 연구진
- 의약학 > 종양의학
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