곽수량 (University of Illinois at Urbana-Champaign)

한빛사논문

조회1,469

University of Illinois at Urbana-Champaign

Biomaterials, Available online 18 January 2022, Volume 282, 121379 | https://doi.org/10.1016/j.biomaterials.2022.121379 Dissection and enhancement of prebiotic properties of yeast cell wall oligosaccharides through metabolic engineering

Suryang Kwak^a,b,1, Scott J. Robinson^c, Jae Won Lee^a,b, Hayoon Lim^a,2, Catherine L. Wallace^c, Yong-Su Jin^a,b,*

^aCarl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL, USA, 61801
^bDepartment of Food Science and Human Nutrition, University of Illinois at Urbana-Champaign, IL, 61801, USA
^cBeckman Institute for Advanced Science & Technology, University of Illinois at Urbana-Champaign, IL, 61801, USA

¹The Edison Family Center for Genome Sciences & Systems Biology, Washington University School of Medicine in St. Louis, MO, USA 63110.
²GC Pharma, Yongin, South Korea 16,924.

^*Corresponding author

Abstract

Saccharomyces boulardii is a yeast clinically used for treating various symptoms of gastrointestinal dysbiosis. Despite their genomic relatedness, S. boulardii has a distinctive cell wall oligosaccharide composition compared to baker's yeast S. cerevisiae, such as higher mannan content. Here we explore the beneficial effects of S. boulardii cell wall oligosaccharides through metabolic engineering. We increased the production of guanosine diphosphate (GDP)-mannose, the substrate for cell wall mannan biosynthesis, by perturbing glycolysis flux and overexpressing the enzymes in the GDP-mannose biosynthesis pathway. Combined with overexpression of a cell wall mannoprotein and dolichol phosphate mannose synthase, the cell wall mannan content of S. boulardii increased up to 52%. The identical engineering resulted in marginal changes in the S. cerevisiae cell wall. S. boulardii showed a higher adhesive capacity against Salmonella enterica Typhimurium than S. cerevisiae, and yeast-bacteria sedimentation rates were positively correlated with cell wall mannan contents. Besides, S. boulardii biomass selectively proliferated Bacteroides thetaiotaomicron over Clostridioides difficile more efficiently than S. cerevisiae, and the selectivity was further enhanced by amplifying the cell wall mannan. Collectively, we report the important prebiotic roles of cell wall oligosaccharides in the protective functions of S. boulardii and present a unique metabolic engineering approach to modulate the functions.

Keywords : Saccharomyces boulardii, Probiotic, Prebiotic, Cell wall oligosaccharide

논문정보

형식Research article
게재일2022년 01월 (BRIC 등록일 2022-01-28)
연구진국외 연구진
분야 생명과학 > 생물공학

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