한빛사논문
Dong Suk Yoon1, Kyoung-Mi Lee1,2, Yoorim Choi1, Eun Ae Ko1, Na-Hyun Lee3,4, Sehee Cho1,5, Kwang Hwan Park1, Jung-Hwan Lee3,4,6,7, Hae-Won Kim3,4,6,7,* and Jin Woo Lee1,2,5,*
1Department of Orthopaedic Surgery, Yonsei University College of Medicine, Seoul 03722, South Korea. 2Severance Biomedical Science Institute, Yonsei University College of Medicine, Seoul 03722, South Korea. 3Institute of Tissue Regeneration Engineering (ITREN), Dankook University, Cheonan 31116, South Korea. 4Department of Nanobiomedical Science and BK21 PLUS NBM Global Research Center for Regenerative Medicine, Dankook University, Cheonan 31116, South Korea. 5Brain Korea 21 PLUS Project for Medical Science, Yonsei University College of Medicine, Seoul 03722, South Korea. 6Department of Biomaterials Science, School of Dentistry, Dankook University, Cheonan 31116, South Korea. 7UCL Eastman-Korea Dental Medicine Innovation Centre, Dankook University, Cheonan 31116, South Korea.
*Correspondence to Hae-Won Kim or Jin Woo Lee.
Abstract
Dysfunction of mRNA or RNA-binding proteins (RBPs) causes cellular aging and age-related degenerative diseases; however, information regarding the mechanism through which RBP-mediated posttranscriptional regulation affects cellular aging and related disease processes is limited. In this study, PUM1 was found to be associated with the self-renewal capacity and aging process of human mesenchymal stem cells (MSC). PUM1 interacted with the 3’-untranslated region of Toll-like receptor 4 (TLR4) to suppress TLR4 mRNA translation and regulate the activity of nuclear factor-κB (NF-κB), a master regulator of the aging process in MSCs. PUM1 overexpression protected MSCs against H2O2-induced cellular senescence by suppressing TLR4-mediated NF-κB activity. TLR4-mediated NF-κB activation is a key regulator in osteoarthritis (OA) pathogenesis. PUM1 overexpression enhanced the chondrogenic potential of MSCs even under the influence of inflammation-inducing factors, such as lipopolysaccharide (LPS) or interleukin-1β (IL-1β), whereas the chondrogenic potential was reduced following the PUM1 knockdown-mediated TLR4 activation. PUM1 levels decreased under inflammatory conditions in vitro and during OA progression in human and mouse disease models. PUM1 knockdown in human chondrocytes promoted chondrogenic phenotype loss, whereas PUM1 overexpression protected the cells from inflammation-mediated disruption of the chondrogenic phenotype. Gene therapy using a lentiviral vector encoding mouse PUM1 showed promise in preserving articular cartilage integrity in OA mouse models. In conclusion, PUM1 is a novel suppressor of MSC aging, and the PUM1-TLR4 regulatory axis represents a potential therapeutic target for OA.
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