Marine organisms react to various factors when building colonies for survival; however, severe accumulation of diverse organisms on artificial structures located close to water causes large industrial losses. Herein, we identify a concept in the development of antifouling surfaces based on understanding the surface stiffness recognition procedure of mussel adhesion at the genetic level. It was found that on a soft surface the combination of decreased adhesive plaque size, adhesion force, and plaque protein downregulation synergistically weakens mussel wet adhesion and sometimes prevents mussels from anchoring, mainly due to transcriptional changes within the mechanosensing pathway and the adhesive proteins in secretory glands. In addition, the use of soft substrates or antagonists of surface mechanosensing behavior suppresses mussel fouling significantly.

KEYWORDS: antifouling, mussel adhesion, mechanosensing, biofouling, antagonist, antagonist-based antifouling