한빛사논문
- 조회653
Liang Sun1,2,3, Jae Won Lee1,2,3, Sangdo Yook1,2,3, Stephan Lane1,2,3, Ziqiao Sun1, Soo Rin Kim4 & Yong-Su Jin1,2,3,*
1Department of Food Science and Human Nutrition, University of Illinois at Urbana-Champaign, Urbana, IL, USA. 2Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL, USA. 3DOE Center for Advanced Bioenergy and Bioproducts Innovation, University of Illinois at Urbana-Champaign, Urbana, IL, USA. 4School of Food Science and Biotechnology, Kyungpook National University, Daegu, South Korea.
*Corresponding author.
Abstract
Plant cell wall hydrolysates contain not only sugars but also substantial amounts of acetate, a fermentation inhibitor that hinders bioconversion of lignocellulose. Despite the toxic and non-consumable nature of acetate during glucose metabolism, we demonstrate that acetate can be rapidly co-consumed with xylose by engineered Saccharomyces cerevisiae. The co-consumption leads to a metabolic re-configuration that boosts the synthesis of acetyl-CoA derived bioproducts, including triacetic acid lactone (TAL) and vitamin A, in engineered strains. Notably, by co-feeding xylose and acetate, an enginered strain produces 23.91 g/L TAL with a productivity of 0.29 g/L/h in bioreactor fermentation. This strain also completely converts a hemicellulose hydrolysate of switchgrass into 3.55 g/L TAL. These findings establish a versatile strategy that not only transforms an inhibitor into a valuable substrate but also expands the capacity of acetyl-CoA supply in S. cerevisiae for efficient bioconversion of cellulosic biomass.
논문정보
- Research article
- 2021년 08월 (BRIC 등록일 2021-11-22)
- 국내+국외 연구진
- 생명과학 > 생물공학
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