한빛사논문
- 조회720
Eun-Song Leea,b, Eun-Ji Kima,1, Tae-Ki Parka, Da-Woon Baec, Sun-Shin Chac, Tae-Wuk Kima,b,d,*, Young-Pil Kima,b,d,e,*
aDepartment of Life Science, Hanyang University, Seoul, 04763, Republic of Korea
bResearch Institute for Convergence of Basic Sciences, Hanyang University, Seoul, 04763, Republic of Korea
cDepartment of Chemistry and Nanoscience, Ewha Womans University, Seoul, 03760, Republic of Korea
dResearch Institute for Natural Sciences, Hanyang University, Seoul, 04763, Republic of Korea
eDepartment of HY-KIST Bio-Convergence, Hanyang University, Seoul, 04763, Republic of Korea
1Current address: VIB-UGENT Center for Plant Systems Biology, Technologiepark 71, 9052 Zwijnaarde, Belgium.
*Corresponding author
Abstract
To resolve time-consuming and imperceptible monitoring problems in the traditional systematic evolution of ligands by exponential enrichment (SELEX), we report gold nanoparticle-assisted SELEX (GNP-SELEX) as a visual, proofreading, and self-monitoring platform and its application to small molecule-binding single-stranded DNA (ssDNA) aptasensors. Through the colorimetric changes between rounds, GNP-SELEX enabled the rapid determination of target-specific aptamer library enrichment with neither target modification nor extra monitoring process. We identified ssDNA aptamers with high selectivity and binding affinity by targeting two small molecules (brassinolide; BL and bisphenol A; BPA) as a model. The rational design of selected aptamers by 3D molecular simulation increased their ability to detect BL or BPA in real samples as bioreceptors. These results suggest that GNP-SELEX is useful as a self-monitoring platform to discover ssDNA aptamers as well as to develop aptasensors for diverse targets in a rapid and simple way.
Keywords : Aptamers, Gold nanoparticle, SELEX, Biosensor, Colorimetry, Brassinolide, Bisphenol
논문정보
- Research article
- 2021년 11월 (BRIC 등록일 2021-11-11)
- 국내 연구진
- 바이오·의료융합 > 바이오센싱 및 나노바이오물질
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