한빛사논문
- 조회1,375
Anh Thi Viet Nguyena,1, Duong Tuan Baoa,1, Hyun Parka, Seon-Ju Yeob,*
aZoonosis Research Center, Department of Infection Biology, School of Medicine, Wonkwang University, Iksan, 54538, South Korea
bDepartment of Tropical Medicine and Parasitology, College of Medicine, Seoul National University, Seoul, 03080, South Korea
1These authors contributed equally to this manuscript.
*Corresponding author.
Abstract
A rapid diagnostic system employing an antigen detection biosensing method is needed to discriminate between Zika virus (ZIKV) and Dengue virus (DENV) due to their close antigenic homology. We developed a novel peptide pair-based flow immunochromatographic test strip (FICT) assay to detect ZIKV. Peptide aptamers, P6.1 (KQERNNWPLTWT), P29.1 (KYTTSTLKSGV), and B2.33 (KRHVWVSLSYSCAEA) were designed by paratopes and modified against the ZIKV envelope protein based on the binding affinity. An antibody-free lateral FICT was developed using a pair of peptide aptamers. In the rapid diagnostic strip, the limit of detection (LOD) for the B2.33-P6.1 peptide pair for ZIKV was 2 × 104 tissue culture infective dose TCID50/mL. Significantly, FICT could discriminate ZIKV from DENV. The stability and performance of FICT were confirmed using human sera and urine, showing a comparable LOD value. Our study demonstrated that in silico modeling could be used to develop a novel peptide pair-based FICT assay for detecting ZIKV by a rapid diagnostic test.
Keywords : Zika virus, Peptide aptamer, Antibody-free peptide pair-linked rapid diagnostic system, Biosensor
논문정보
- Research article
- 2021년 11월 (BRIC 등록일 2021-11-22)
- 국내 연구진
댓글 작성 로그인
팝업 닫기관련 링크
연구자 키워드
연구자 ID
관련분야 연구자보기
소속기관 논문보기
관련분야 논문보기
