한빛사논문
- 조회573
Soon Hee Kim1,7, Heesun Hong1,7, Olatunji Ajiteru1,7, Md. Tipu Sultan1,7, Young Jin Lee1, Ji Seung Lee1, Ok Joo Lee1, Hanna Lee1, Hae Sang Park1,2, Kyu Young Choi1,3, Joong Seob Lee1,4, Hyung Woo Ju5, In-Sun Hong6 and Chan Hum Park1,2,*
1Nano-Bio Regenerative Medical Institute, College of Medicine, Hallym University, Chuncheon, Republic of Korea. 2Departments of Otorhinolaryngology-Head and Neck Surgery, Chuncheon Sacred Heart Hospital, School of Medicine, Hallym University, Chuncheon, Republic of Korea. 3Department of Otorhinolaryngology, Kangnam Sacred Heart Hospital, Hallym University College of Medicine, Seoul, Republic of Korea. 4Department of Otorhinolaryngology, Hallym University Sacred Heart Hospital, Anyang, Republic of Korea. 5Nano-Bio Regenerative Technology Company Ltd., Chuncheon, Republic of Korea. 6Department of Molecular Medicine, School of Medicine, Gachon University, Incheon, Republic of Korea. 8These authors contributed equally: Soon Hee Kim, Heesun Hong, Olatunji Ajiteru, Md. Tipu Sultan.
*Corresponding author.
Abstract
The development of biocompatible and precisely printable bioink addresses the growing demand for three-dimensional (3D) bioprinting applications in the field of tissue engineering. We developed a methacrylated photocurable silk fibroin (SF) bioink for digital light processing 3D bioprinting to generate structures with high mechanical stability and biocompatibility for tissue engineering applications. Procedure 1 describes the synthesis of photocurable methacrylated SF bioink, which takes 2 weeks to complete. Digital light processing is used to fabricate 3D hydrogels using the bioink (1.5 h), which are characterized in terms of methacrylation, printability, mechanical and rheological properties, and biocompatibility. The physicochemical properties of the bioink can be modulated by varying photopolymerization conditions such as the degree of methacrylation, light intensity, and concentration of the photoinitiator and bioink. The versatile bioink can be used broadly in a range of applications, including nerve tissue engineering through co-polymerization of the bioink with graphene oxide, and for wound healing as a sealant. Procedure 2 outlines how to apply 3D-printed SF hydrogels embedded with chondrocytes and turbinate-derived mesenchymal stem cells in one specific in vivo application, trachea tissue engineering, which takes 2–9 weeks.
논문정보
- Research article
- 2021년 10월 (BRIC 등록일 2021-11-05)
- 국내 연구진
- 바이오·의료융합 > 바이오소재
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