강태준 (한국생명공학연구원)

한빛사논문

조회517

한국생명공학연구원

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Biosens. Bioelectron., Available online 26 August 2021, Volume 194, 13593 | https://doi.org/10.1016/j.bios.2021.113593 Development of antibody against drug-resistant respiratory syncytial virus: Rapid detection of mutant virus using split superfolder green fluorescent protein-antibody system

Hyeran Kim^a, Seul Gee Hwang^a, Kyeonghye Guk^a, Yoonji Bae^a, Hwangseo Park^b, Eun-Kyung Lim^a,c,*, Taejoon Kang^a,*, Juyeon Jung^a,*

^aBionanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, 34141, Republic of Korea
^bDepartment of Bioscience and Biotechnology, Sejong University, Seoul, 05006, Republic of Korea
^cDepartment of Nanobiotechnology, KRIBB School of Biotechnology, University of Science and Technology (UST), Daejeon, 34113, Republic of Korea

^*Corresponding author

Abstract

Respiratory syncytial virus (RSV) infections are associated with severe bronchiolitis or pneumonia. Although palivizumab is used to prevent RSV infections, the occurrence of palivizumab-resistant RSV strains is increasing, and these strains pose a threat to public health. Herein, we report an antibody with affinity to the S275F RSV antigen, enabling the specific detection of palivizumab-resistant RSV strains. Experimental and simulation results confirmed the affinity of the antibody to the S275F RSV antigen. Furthermore, we developed a rapid S275F RSV antigen detection method using a split superfolder green fluorescent protein (ssGFP) that can interact with the antibody. In the presence of the mutant virus antigen, ssGFP emitted fluorescence within 1 min, allowing the rapid identification of S275F RSV. We anticipate that the developed antibody would be useful for the precise diagnosis of antiviral drug-resistant RSV strains and help treat patients with RSV infections.

Keywords : Antibody, Drug-resistance, S275F mutation, Respiratory syncytial virus, Green fluorescent protein

논문정보

형식Research article
게재일2021년 08월 (BRIC 등록일 2021-11-03)
연구진국내 연구진
분야 바이오·의료융합 > 바이오센싱 및 나노바이오물질

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