한빛사논문
- 조회568
고려대학교
Hong Il Choi1,4, Sung-Won Hwang1,4, Jongrae Kim2, Byeonghyeok Park3, EonSeon Jin2, In-Geol Choi3 & Sang Jun Sim1,*
1Department of Chemical and Biological Engineering, Korea University, 145, Anam-ro, Seongbuk-gu, Seoul 02841, Republic of Korea. 2Department of Life Science, Hanyang University, 206, Wangsimni-ro, Seongbuk-gu, Seoul 04763, Republic of Korea. 3Department of Biotechnology, Korea University, 145, Anam-ro, Seongbuk-gu, Seoul 02841, Republic of Korea. 4These authors contributed equally: Hong Il Choi, Sung-Won Hwang.
*Corresponding author.
Abstract
Microalgae can accumulate various carbon-neutral products, but their real-world applications are hindered by their CO2 susceptibility. Herein, the transcriptomic changes in a model microalga, Chlamydomonas reinhardtii, in a high-CO2 milieu (20%) are evaluated. The primary toxicity mechanism consists of aberrantly low expression of plasma membrane H+-ATPases (PMAs) accompanied by intracellular acidification. Our results demonstrate that the expression of a universally expressible PMA in wild-type strains makes them capable of not only thriving in acidity levels that they usually cannot survive but also exhibiting 3.2-fold increased photoautotrophic production against high CO2 via maintenance of a higher cytoplasmic pH. A proof-of-concept experiment involving cultivation with toxic flue gas (13 vol% CO2, 20 ppm NOX, and 32 ppm SOX) shows that the production of CO2-based bioproducts by the strain is doubled compared with that by the wild-type, implying that this strategy potentially enables the microalgal valorization of CO2 in industrial exhaust.
논문정보
- Research article
- 2021년 10월 (BRIC 등록일 2021-10-29)
- 국내 연구진
- 생명과학 > 생물공학
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