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기초과학연구원, 연세대학교

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Biosens. Bioelectron., Volume 178, 15 April 2021, 113049 | https://doi.org/10.1016/j.bios.2021.113049 Fluorescence polarization system for rapid COVID-19 diagnosis

Chang Yeol Lee^a,b,1, Ismail Degani^b,c,1, Jiyong Cheong^a,d, Jae-Hyun Lee^a,d, Hyun-Jung Choi^e,*, Jinwoo Cheon^a,d,f,*, Hakho Lee^a,b,d,g,*

^aInstitute for Basic Science (IBS), Center for Nanomedicine, Seoul, South Korea
^bCenter for Systems Biology, Massachusetts General Hospital Research Institute, Boston, MA, USA
^cDepartment of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, Cambridge, MA, USA
^dGraduate Program of Nano Biomedical Engineering (NanoBME), Advanced Science Institute, Yonsei University, Seoul, South Korea
^eDepartment of Laboratory Medicine, Chonnam National University Medical School and Chonnam National University Hospital, Gwangju, South Korea
^fDepartment of Chemistry, Yonsei University, Seoul, South Korea
^gDepartment of Radiology, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA

¹These authors contributed equally.

^*Corresponding author

Abstract

Prompt diagnosis, patient isolation, and contact tracing are key measures to contain the coronavirus disease 2019 (COVID-19). Molecular tests are the current gold standard for COVID-19 detection, but are carried out at central laboratories, delaying treatment and control decisions. Here we describe a portable assay system for rapid, onsite COVID-19 diagnosis. Termed CODA (CRISPR Optical Detection of Anisotropy), the method combined isothermal nucleic acid amplification, activation of CRISPR/Cas12a, and signal generation in a single assay, eliminating extra manual steps. Importantly, signal detection was based on the ratiometric measurement of fluorescent anisotropy, which allowed CODA to achieve a high signal-to-noise ratio. For point-of-care operation, we built a compact, standalone CODA device integrating optoelectronics, an embedded heater, and a microcontroller for data processing. The developed system completed SARS-CoV-2 RNA detection within 20 min of sample loading; the limit of detection reached 3 copy/μL. When applied to clinical samples (10 confirmed COVID-19 patients; 10 controls), the rapid CODA test accurately classified COVID-19 status, in concordance with gold-standard clinical diagnostics.

Keywords : Biosensor, COVID-19, CRISPR/Cas, Isothermal amplification, Fluorescence polarization, Point-of-care

논문정보

형식Research article
게재일2021년 04월 (BRIC 등록일 2021-09-17)
연구진국내(교신)+국외 연구진
분야 바이오·의료융합 > 바이오센싱 및 나노바이오물질

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