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농림축산검역본부

Transbound. Emerg. Dis., First published: 01 September 2021 | https://doi.org/10.1111/tbed.14309 In-depth examination of PrPSc in Holstein cattle carrying the E211K somatic mutation of the bovine prion protein gene (PRNP)

Yong-Chan Kim^1,2, Kyung-Je Park³, Ji-Yong Hwang³, Hoo-Chang Park³, Hae-Eun Kang³, Hyun-Joo Sohn^3,*, Byung-Hoon Jeong^1,2,*

¹Korea Zoonosis Research Institute, Jeonbuk National University, Iksan, Republic of Korea
²Department of Bioactive Material Sciencesand Institute for Molecular Biology and Genetics, Jeonbuk National University, Jeonju, Republic of Korea
³Reference Laboratory for CWD, Foreign Animal Disease Division, Animal and Plant Quarantine Agency, Gimcheon, Republic of Korea

^*Correspondence :
Byung-Hoon Jeong, Korea Zoonosis ResearchInstitute, Jeonbuk National University, 820-120, Hana-ro, Iksan, Jeonbuk 54531, Republicof Korea

Hyun-Joo Sohn, Reference Laboratory for CWD, ForeignAnimal Disease Division, Animal and Plant Quarantine Agency, Gimcheon, 39660, Republic of Korea

Abstract

Prion diseases are transmissible spongiform encephalopathies caused by deleterious prion protein (PrPSc) derived from normal prion protein (PrPC), which is encoded by the prion protein gene (PRNP). We performed an in-depth examination to detect PrPSc by using enzyme immunoassay (EIA), real-time quaking-induced conversion reactions (RT-QuIC) and protein misfolding cyclic amplification (PMCA) in nine brain tissues derived from three Holstein cattle carrying the E211K somatic mutation of the bovine PRNP gene. The EIA, RT-QuIC and PMCA analyses were not able to detect the PrPSc band in any tested samples. To the best of our knowledge, this report is the first to describe an in-depth examination of PrPSc in cattle carrying the E211K somatic mutation of the bovine PRNP gene.

논문정보

형식Research article
게재일2021년 09월 (BRIC 등록일 2021-09-27)
연구진국내 연구진
분야 의약학 > 감염의학
구분구・농수식품

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