한빛사논문
- 조회536
Min Gao,[a]■ Sun Hyeok Lee,[b]■ Sang Hyuk Park,[d] Larissa Miasiro Ciaramicoli,[c] Haw-Young Kwon,[a] Heewon Cho,[b] Joseph Jeong,[d] Young-Tae Chang*[a][b][c]
[a] Center for Self-assembly and Complexity, Institute for Basic Science (IBS), Pohang 37673, Republic of Korea.
[b] School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology (POSTECH), Pohang 37673, Republic of Korea
[c] Department of Chemistry, Pohang University of Science and Technology (POSTECH), Pohang 37673, Republic of Korea.
[d] Department of Laboratory Medicine, University of Ulsan College of Medicine (UUCM), Ulsan University Hospital, Ulsan 44033, Republic of Korea
■M. Gao and S. H. Lee contributed equally.
*Corresponding author.
Abstract
Human neutrophils are the most abundant leukocytes and have been considered as the first line of defence in the innate immune system. Selective imaging of live neutrophils will facilitate in situ study of neutrophils in infection or inflammation event as well as clinical diagnosis. However, the small-molecule-based probe is absent for the discrimination of live neutrophils among different granulocytes in human blood. Herein, we report the first fluorescent probe NeutropG for the specific distinction and imaging of active neutrophils. The selective staining mechanism of NeutropG is elucidated as Metabolism-Oriented Live-cell Distinction (MOLD) through lipid droplet biogenesis with the help of ACSL and DGAT. Finally, NeutropG is applied to accurately quantify neutrophil levels in fresh blood samples by showing a high correlation with the current clinical method.
논문정보
- Research article
- 2021년 08월 (BRIC 등록일 2021-09-01)
- 국내 연구진
- 바이오·의료융합 > 바이오센싱 및 나노바이오물질
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