한빛사논문
Doyeon Kim1,6, Sukjun Kim1,6, Joori Park2,3,6, Hee Ryung Chang1,6, Jeeyoon Chang2,3,6, Junhak Ahn1,6, Heedo Park4,6, Junehee Park1, Narae Son1, Gihyeon Kang1, Jeonghun Kim4, Kisoon Kim4, Man-Seong Park4,*, Yoon Ki Kim2,3,* & Daehyun Baek1,5,*
1School of Biological Sciences, Seoul National University, Seoul, Republic of Korea. 2Creative Research Initiatives Center for Molecular Biology of Translation, Korea University, Seoul, Republic of Korea. 3Division of Life Sciences, Korea University, Seoul, Republic of Korea. 4Department of Microbiology, Institute for Viral Diseases, College of Medicine, Korea University, Seoul, Republic of Korea. 5Bioinformatics Institute, Seoul National University, Seoul, Republic of Korea. 6These authors contributed equally: Doyeon Kim, Sukjun Kim, Joori Park, Hee Ryung Chang, Jeeyoon Chang, Junhak Ahn, Heedo Park.
*Corresponding author.
Abstract
COVID-19 is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which infected >200 million people resulting in >4 million deaths. However, temporal landscape of the SARS-CoV-2 translatome and its impact on the human genome remain unexplored. Here, we report a high-resolution atlas of the translatome and transcriptome of SARS-CoV-2 for various time points after infecting human cells. Intriguingly, substantial amount of SARS-CoV-2 translation initiates at a novel translation initiation site (TIS) located in the leader sequence, termed TIS-L. Since TIS-L is included in all the genomic and subgenomic RNAs, the SARS-CoV-2 translatome may be regulated by a sophisticated interplay between TIS-L and downstream TISs. TIS-L functions as a strong translation enhancer for ORF S, and as translation suppressors for most of the other ORFs. Our global temporal atlas provides compelling insight into unique regulation of the SARS-CoV-2 translatome and helps comprehensively evaluate its impact on the human genome.
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