한빛사논문
- 조회846
성균관대학교, 현 Wake Forest Institute for Regenerative Medicine
WonJin Kim, Hyeongjin Lee, Chang Kyu Lee, Jae Won Kyung, Seong Bae An, In-Bo Han,* and Geun Hyung Kim*
W. Kim, H. Lee, G. H. Kim
Department of Biomechatronic Engineering
College of Biotechnology and Bioengineering
Sungkyunkwan University
Suwon 16419, South Korea
C. K. Lee
Department of Neurosurgery, Spine and Spinal Cord Institute
Severance Hospital
Yonsei University College of Medicine
Seoul 03722, South Korea
J. W. Kyung, S. B. An, I.-B. Han
Department of Neurosurgery
CHA University School of Medicine
CHA Bundang Medical Center
Seongnam-si 13497, South Korea
G. H. Kim
Biomedical Institute for Convergence at SKKU (BICS)
Sungkyunkwan University
Suwon 16419, Republic of Korea
W.K., H.L., and C.K.L. contributed equally to this work.
*Corresponding author
Abstract
Electric field stimulation has supported biophysical and biological cues for tissue regeneration approaches to affect cell morphology, alignment, and even cellular phenotypes types. Here, an innovative bioprinting approach supported by in situ electrical stiumlation (E-printing) is used to fabricate a bioengineered skeletal muscle construct composed of human adipose stem cells and methacrylated decellularized extracellular matrix (dECM-Ma) derived from porcine muscle. To obtain highly ordered myofiber-like structures, various parameters of the printing process are optimized. The E-printed structure exhibits higher cell viability and fully aligned cytoskeleton than the conventionally printed cell-bearing structures, due to activation of voltage-gated ion channels that affect various signaling pathways. When using the E-printed structure, expression of myogenesis-related genes is upregulated by 1.9–2.5-fold higher than when using a dECM-Ma structure produced without electrical stimulation. Furthermore, when implanted into a rat model of volumetric muscle loss, the structure yields outstanding myogenesis relative to the conventionally bioprinted structure.
논문정보
- Research article
- 2021년 08월 (BRIC 등록일 2021-08-25)
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