한빛사논문
- 조회450
Sungeun Leea, Ella T. Sieradzkib, Alexa M. Nicolasc, Robin L. Walkerd, Mary K. Firestoneb,e, Christina Hazarda,1, and Graeme W. Nicola,1,2
aEnvironmental Microbial Genomics Group, Laboratoire Ampère, École Centrale de Lyon, CNRS UMR 5005, Université de Lyon, Ecully 69134, France;
bDepartment of Environmental Science, Policy and Management, University of California, Berkeley, CA 94720;
cDepartment of Plant and Microbial Biology, University of California, Berkeley, CA 94720;
dSchool of Rural Land Use, Scotland's Rural College, Aberdeen, AB21 9YA, United Kingdom;
eEarth Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720
1C.H. and G.W.N. contributed equally to this work.
2To whom correspondence may be addressed.
Abstract
The concentration of atmospheric methane (CH4) continues to increase with microbial communities controlling soil–atmosphere fluxes. While there is substantial knowledge of the diversity and function of prokaryotes regulating CH4 production and consumption, their active interactions with viruses in soil have not been identified. Metagenomic sequencing of soil microbial communities enables identification of linkages between viruses and hosts. However, this does not determine if these represent current or historical interactions nor whether a virus or host are active. In this study, we identified active interactions between individual host and virus populations in situ by following the transfer of assimilated carbon. Using DNA stable-isotope probing combined with metagenomic analyses, we characterized CH4-fueled microbial networks in acidic and neutral pH soils, specifically primary and secondary utilizers, together with the recent transfer of CH4-derived carbon to viruses. A total of 63% of viral contigs from replicated soil incubations contained homologs of genes present in known methylotrophic bacteria. Genomic sequences of 13C-enriched viruses were represented in over one-third of spacers in CRISPR arrays of multiple closely related Methylocystis populations and revealed differences in their history of viral interaction. Viruses infecting nonmethanotrophic methylotrophs and heterotrophic predatory bacteria were also identified through the analysis of shared homologous genes, demonstrating that carbon is transferred to a diverse range of viruses associated with CH4-fueled microbial food networks.
논문정보
- Research article
- 2021년 08월 (BRIC 등록일 2021-08-24)
- 국외 연구진
- 생명과학 > 미생물학
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