한빛사논문
- 조회416
성균관대학교
Hanjun Hwangboa,1, Hyeongjin Leea,1, Eun-Ju Jinb,1, JaeYoon Leea, Yunju Job, Dongryeol Ryub,c,*, GeunHyung Kima,c,*
aDepartment of Biomechatronics Engineering, College of Biotechnology and Bioengineering, Sungkyunkwan University (SKKU), Suwon, 16419, Republic of Korea
bDepartment of Molecular Cell Biology, Sungkyunkwan University School of Medicine (SKKU-SOM), Suwon, 16419, Republic of Korea
cBiomedical Institute for Convergence at SKKU (BICS), Sungkyunkwan University, Suwon, 16419, Republic of Korea
*Corresponding author.
Abstract
Volumetric muscle loss (VML) is associated with a severe loss of muscle tissue that overwhelms the regenerative potential of skeletal muscles. Tissue engineering has shown promise for the treatment of VML injuries, as evidenced by various preclinical trials. The present study describes the fabrication of a cell-laden GelMa muscle construct using an in situ crosslinking (ISC) strategy to improve muscle functionality. To obtain optimal biophysical properties of the muscle construct, two UV exposure sources, UV exposure dose, and wall shear stress were evaluated using C2C12 myoblasts. Additionally, the ISC system showed a significantly higher degree of uniaxial alignment and myogenesis compared to the conventional crosslinking strategy (post-crosslinking). To evaluate the in vivo regenerative potential, muscle constructs laden with human adipose stem cells were used. The VML defect group implanted with the bio-printed muscle construct showed significant restoration of functionality and muscular volume. The data presented in this study suggest that stem cell-based therapies combined with the modified bioprinting process could potentially be effective against VML injuries.
논문정보
- Research article
- 2021년 07월 (BRIC 등록일 2021-08-12)
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