한빛사논문
- 조회649
Dongkyu Kanga,d, Hyun Joo Ahnb, Jiho Leec, Sang Kyung Kima, Jaechul Pyund, Chang-Seon Songc, Sang Jick Kimb,*, Joonseok Leea,e,*
aMolecular Recognition Research Center, Korea Institute of Science and Technology (KIST), Seoul, 02792, South Korea
bSynthetic Biology and Bioengineering Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, 34141, Republic of Korea
cAvian Disease and Infectious Disease Laboratory, College of Veterinary Medicine, Konkuk University, Seoul, 05029, South Korea
dDepartments of Material Science and Engineering, Yonsei University, Seodaemun-gu, Seoul, 120-749, South Korea
eDepartment of Chemistry, Hanyang University, Seoul, 04763, Republic of Korea
* Corresponding author.
Abstract
Many conventional optical biosensing systems use a single responsive signal in the visible light region. This limits their practical applications, as the signal can be readily perturbed by various external environmental factors. Herein, a near-infrared (NIR)-based self-calibrating luminescence resonance energy transfer (LRET) system was developed for background-free detection of analytes in homogeneous sandwich-immunoassays. The inorganic LRET pair was comprised of NIR dual-emitting lanthanide-doped nanoparticles (LnNPs) as donors and NIR-absorbing LnNPs as acceptors, which showed a narrow absorption peak (800 nm) and long-term stability, enabling stable LRET with a built-in self-calibrating signal. Screened single-chain variable fragments (scFvs) were used as target avian influenza virus (AIV)-binding antibodies to increase the LRET efficiency in sandwich-immunoassays. The compact sensor platform successfully detected AIV nucleoproteins with a 0.38 pM limit of detection in buffer solution and 64 clinical samples. Hence, inorganic LnNP pairs may be effective for self-calibrating LRET systems in the background-free NIR region.
논문정보
- Research article
- 2021년 10월 (BRIC 등록일 2021-08-17)
- 국내 연구진
- 바이오·의료융합 > 바이오센싱 및 나노바이오물질
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