김석형 (성균관대학교 의과대학, 삼성서울병원 병리과) | 한빛사논문 > 한빛사

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성균관대학교 의과대학, 삼성서울병원 병리과

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Clin. Transl. Med., First published: 19 July 2021 | https://doi.org/10.1002/ctm2.487 Integrated single-cell RNA sequencing analyses suggest developmental paths of cancer-associated fibroblasts with gene expression dynamics

Hee Chul Chung^1,2, Eun Jeong Cho^1,3, Hyeonjin Lee^1,2, Won-Kyung Kim^1,3, Ji-Hye Oh^1,3, Seok-Hyung Kim^4,*, Dakeun Lee^5,*, Chang Ohk Sung^1,2,3,*

¹Center for Cancer Genome Discovery, Asan Institute for Life Sciences, Asan Medical Center, Songpa-gu, Seoul, Republic of Korea
²Department of Pathology, Asan Medical Center, University of Ulsan College of Medicine, Songpa-gu, Seoul, Republic of Korea
³Department of Medical Science, Asan Medical Institute of Convergence Science and Technology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Republic of Korea
⁴Department of Pathology and Translational Genomics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Republic of Korea
⁵Department of Pathology, Ajou University School of Medicine, Suwon, Republic of Korea

^*Correspondence :
Chang Ohk Sung, MD, PhD, Department of Pathology, Asan Medical Center, University of Ulsan College of Medicine, 88 Olympic-ro 43-gil, Songpa-gu, Seoul 05505, Republic of Korea.

Dakeun Lee, MD, PhD, Department of Pathology, Ajou University School of Medicine, 164, Worldcup-ro, Yeongtong-gu, Suwon-si, Gyeonggi-do 16499, Republic of Korea.

Seok-Hyung Kim, MD, PhD, Department of Pathology, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul 06351, Republic of Korea

Abstract

Dear Editor,
The origin and the phenotypic heterogeneity of cancer-associated fibroblasts (CAFs) are suggested by various models, but not completely understood.1-3 We used six publicly available single-cell RNA sequencing (scRNA-seq) datasets of five cancer types (except breast cancer) on CAFs and corresponding normal fibroblasts (NFs) (Figure 1A)4-6 and established a comprehensive model for CAF development and gene expression dynamics over time. The fibroblast fraction constituted less than 10% of all cellular components in each dataset (Figure 1B). This relatively low fraction may be ascribed to our two-step, strict procedure for defining fibroblasts.

논문정보

형식Research article
게재일2021년 07월 (BRIC 등록일 2021-07-21)
연구진국내 연구진
분야 의약학 > 응용의학

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