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J. Hazard. Mater., Volume 414, 15 July 2021, 125472 | https://doi.org/10.1016/j.jhazmat.2021.125472 2D graphene oxide particles induce unwanted loss in pluripotency and trigger early differentiation in human pluripotent stem cells

Jiwoong Heo^a,1, Jaewon Choi^b,1, Jin Young Kim^c, Hyejoong Jeong^a, Daheui Choi^a, Uiyoung Han^a, Ju Hyun Park^d, Hee Ho Park^b,c,*, Jinkee Hong^a,*

^aDepartment of Chemical and Biomolecular Engineering, College of Engineering, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul 03722, Republic of Korea
^bInterdisciplinary Program in Biohealth-Machinery Convergence Engineering, Kangwon National University, Chuncheon, Gangwon-do 24341, Republic of Korea
^cDepartment of Biotechnology and Bioengineering, Kangwon National University, Chuncheon, Gangwon-do 24341, Republic of Korea
^dDepartment of Biomedical Science, Kangwon National University, Chuncheon, Gangwon-do 24341, Republic of Korea

¹Jiwoong Heo and Jaewon Choi contributed equally to this work.

^*Corresponding author.

Abstract

The potential health hazards of particulates, such as micro/nano-sized plastics and carbon materials have recently received extensive attention. However, their toxicological properties in association with stem cell differentiation is still relatively unexplored. In this study, we elucidated the cytotoxic effects of 2D graphene oxide (GO), in relation to differentiation of human induced pluripotent stem cells (hiPSCs). Supplementation of GO to hiPSCs demonstrated uptake of GO through the plasma membrane and intracellular accumulation was observed. Increasing the concentration of GO led to reduced viability and increased likelihood of hiPSC colony detachment. Moreover, treatment of GO resulted in significant loss in pluripotency markers, OCT-4 and NANOG. In particular, when hiPSCs were cultured with GO in cardiomyocyte induction medium, upregulation of cardiomyocyte marker, NKX2.5, along with observation of early triggering of differentiation were observed. Taken together, our results highlight the risk in the uptake and accumulation of GO on the stem cell development by unwanted loss in pluripotency and accelerated initiation of differentiation.

Keywords : 2D graphene oxide, hiPSC, Cytotoxicity, Pluripotency, Unwanted differentiation, Early triggering of differentiation

논문정보

형식Research article
게재일2021년 07월 (BRIC 등록일 2021-07-20)
연구진국내 연구진
분야 바이오·의료융합 > 바이오소재

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