이조한 (POSTECH)

한빛사논문

조회208

이조한Johan Yi

POSTECH

ACS Nano, Publication Date:June 28, 2021 | https://doi.org/10.1021/acsnano.1c02556 Multifluorescence Single Extracellular Vesicle Analysis by Time-Sequential Illumination and Tracking

Siwoo Cho^1,3,‡, Johan Yi^1,‡, Yongmin Kwon¹, Hyejin Kang², Chungmin Han¹, Jaesung Park^1,2,*

¹Department of Mechanical Engineering, Pohang University of Science and Technology, 77 Cheongam-Ro. Nam-Gu, Pohang, Gyeong-buk, Republic of Korea 37673
²School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology, 77 Cheongam-Ro. Nam-Gu, Pohang, Gyeong-buk, Republic of Korea 37673
³EXOSOMEplus Inc., Suwon,Gyeonggi-do, Republic of Korea 16229

^*Correspondence:Jaesung Park
^‡These authors contributed equally

Abstract

We demonstrate a fluorescence-based nanoparticle tracking analysis (NTA) system for the characterization of both the size and membrane protein expression of individual extracellular vesicles (EVs). A sheet of lasers with four different wavelengths was sequentially shone onto extracellular vesicles according to a preprogrammed schedule, providing scattering images intercalated by three fluorescent images. The presence of extracellular vesicles was tracked frame by frame from scattering images. Fluorescence-labeled membrane proteins on EVs were detected by comparing scattering and fluorescent images. The tetraspanins (CD9, CD63, and CD81) of individual HEK293 EVs analyzed by both NTA and total internal reflection fluorescence microscopy showed that the proposed NTA system can contribute to the understanding of individual extracellular vesicles.

KEYWORDS : nanoparticle, extracellular vesicle, nanoparticle tracking analysis, time-sequential illumination, multifluorescence

논문정보

형식Research article
게재일2021년 06월 (BRIC 등록일 2021-07-09)
연구진국내 연구진
분야 바이오·의료융합 > 바이오센싱 및 나노바이오물질

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