구.농수식품
Beom-Soon Choia,1, Duck-Hyun Kimb,1, Min-Sub Kimb,1, Jun Chul Parkb,1, Young Hwan Leeb, Hee-Jin Kimc, Chang-Bum Jeongd, Atsushi Hagiwarac,e, Sami Souissif, Jae-Seong Leeb,*
aPhyzen Genomics Institute, Seoul 08787, South Korea
bDepartment of Biological Sciences, College of Science, Sungkyunkwan University, Suwon 16419, South Korea
cGraduate School of Fisheries and Environmental Sciences, Nagasaki University, Nagasaki 852-8521, Japan
dDepartment of Marine Science, College of Nature Science, Incheon National University, Incheon 22012, South Korea
eOrganization for Marine Science and Technology, Nagasaki University, Nagasaki 852-8521, Japan
fUniversité de Lille, CNRS, Université du Littoral Côte d'Opale, UMR 8187 LOG, Laboratoire d'Océanologie et de Géosciences, Station marine de Wimereux, F-59000 Lille, France
1These authors contributed equally to this work.
*Corresponding author.
Abstract
In this study, we sequenced and assembled the genome of a European estuarine calanoid copepod using Oxford Nanopore PromethION and Illumina HiSeq 2500 platforms. The length of the assembled genome was 776.1 Mb with N50 = 474.9 kb (BUSCO 85.9%), and the genome consisted of 2473 contigs. A total of 18,014 genes were annotated and orthologous gene clusters were analyzed in comparison to other copepods. In addition, genome-wide identification of cytochrome P450s, glutathione S-transferases, and ATP-binding cassette transporters in E. affinis was performed to determine gene repertoire of these detoxification-related gene families. Results revealed the presence of species-specific gene inventories, indicating that these gene families have evolved through species-specific gene loss/expansion processes, possibly due to adaptation to different environmental stressors. Our study provides a new inventory of the European estuarine calanoid copepod E. affinis genome with emphasis on phase I, II, and III detoxification systems.
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