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Adv. Funct. Mater., First published: 18 June 2021 | https://doi.org/10.1002/adfm.202103409 Immunoregulation of Macrophages by Controlling Winding and Unwinding of Nanohelical Ligands

Gunhyu Bae^a, Yoo Sang Jeon^a, Min Jun Ko^a, Yuri Kim^a, Seong-Beom Han^c, Ramar Thangam^a, Wonsik Kim^d, Hee Joon Jung^e,f,g, Sungkyu Lee^a, Hyojun Choi^a, Sunhong Min^a,
Hyunsik Hong^a, Sangwoo Park^a,d,h, Seong Yeol Kim^a,h, Kapil D. Patel^a, Na Liⁱ, Jeong Eun Shin^a, Bum Chul Park^a, Hyeon Su Park^a, Jun Hwan Moon^a, Yu Jin Kim^a, Uday Kumar Sukumar^j, Jae-Jun Songⁱ, Soo Young Kim^a, Seung-Ho Yu^k, Yun Chan Kang^a, Steve Park^d, Seung Min Han^d, Dong-Hwee Kim^c, Ki-Bum Lee^l, Qiang Wei^m, Liming Biaⁿ, Ramasamy Paulmurugan^j,n, Young Keun Kim^a,* and Heemin Kang^a,b,*

^aDepartment of Materials Science and Engineering, Korea University, Seoul 02841, Republic of Korea
^bDepartment of Biomicrosystem Technology, Korea University, Seoul 02841, Republic of Korea
^cKU-KIST Graduate School of Converging Science and Technology, Korea University, Seoul 02841, Republic of Korea
^dDepartment of Materials Science and Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 34141, Republic of Korea
^eDepartment of Materials Science and Engineering, Northwestern University, Evanston, IL 60208, USA
^fInternational Institute for Nanotechnology, Evanston, IL 60208, USA
^gNUANCE Center, Northwestern University, Evanston, IL 60208, USA
^hInstitute for High Technology Materials and Devices, Korea University, Seoul 02841, Republic of Korea
ⁱDepartment of Otorhinolaryngology-Head and Neck Surgery, Korea University College of Medicine, Seoul 08308, Republic of Korea
^jDepartment of Radiology, Molecular Imaging Program at Stanford, Stanford University School of Medicine, Stanford University, Palo Alto, CA 94304, USA
^kDepartment of Chemical and Biological Engineering, Korea University, Seoul 02841, Republic of Korea
^lDepartment of Chemistry and Chemical Biology, Rutgers University, Piscataway, NJ 08854, USA
^mCollege of Polymer Science and Engineering, State Key Laboratory of Polymer Materials and Engineering, Sichuan University, Chengdu 610065, China
ⁿDepartment of Radiology, Canary Center at Stanford for Cancer Early Detection, Stanford University School of Medicine, Stanford University, Palo Alto, CA 94304, USA

G.B. and Y.S.J. contributed equally to this work.

^*To whom correspondence should be addressed.

Abstract

Developing materials with the capability of changing their innate features can help to unravel direct interactions between cells and ligand-displaying features. This study demonstrates the grafting of magnetic nanohelices displaying cell-adhesive Arg-Gly-Asp (RGD) ligand partly to a material surface. These enable nanoscale control of rapid winding (“W”) and unwinding (“UW”) of their nongrafted portion, such as directional changes in nanohelix unwinding (lower, middle, and upper directions) by changing the position of a permanent magnet while keeping the ligand-conjugated nanohelix surface area constant. The unwinding (“UW”) setting cytocompatibility facilitates direct integrin recruitment onto the ligand-conjugated nanohelix to mediate the development of paxillin adhesion assemblies of macrophages that stimulate M2 polarization using glass and silicon substrates for in vitro and in vivo settings, respectively, at a single cell level. Real time and in vivo imaging are demonstrated that nanohelices exhibit reversible unwinding, winding, and unwinding settings, which modulate time-resolved adhesion and polarization of macrophages. It is envisaged that this remote, reversible, and cytocompatible control can help to elucidate molecular-level cell–material interactions that modulate regenerative/anti-inflammatory immune responses to implants.

논문정보

형식Research article
게재일2021년 06월 (BRIC 등록일 2021-06-28)
연구진국내(교신)+국외 연구진
분야 바이오·의료융합 > 바이오센싱 및 나노바이오물질

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