정상화 (부산대학교, University of California, Berkeley)

한빛사논문

조회670

부산대학교, University of California, Berkeley

CV File 230 kb

ACS Nano, Publication Date:June 9, 2021 | https://doi.org/10.1021/acsnano.1c02494 Extraction of Viral Nucleic Acids with Carbon Nanotubes Increases SARS-CoV-2 Quantitative Reverse Transcription Polymerase Chain Reaction Detection Sensitivity

Sanghwa Jeong^1,2, Eduardo González-Grandío¹, Nicole Navarro³, Rebecca L. Pinals¹, Francis Ledesma¹, Darwin Yang¹, and Markita P. Landry^*1,4-6

¹Department of Chemical and Biomolecular Engineering, University of California, Berkeley, California 94720, United States
²School of Biomedical Convergence Engineering, Pusan National University, Yangsan, 50612, Republic of Korea
³Department of Chemistry, University of California, Berkeley, California 94720, United States
⁴Innovative Genomics Institute (IGI), Berkeley, California 94720, United States
⁵California Institute for Quantitative Biosciences, QB3, University of California, Berkeley, California 94720, United States
⁶Chan-Zuckerberg Biohub,San Francisco, California 94158, United States

^*Corresponding Author

Abstract

The global SARS-CoV-2 coronavirus pandemic has led to a surging demand for rapid and efficient viral infection diagnostic tests, generating a supply shortage in diagnostic test consumables including nucleic acid extraction kits. Here, we develop a modular method for high-yield extraction of viral single-stranded nucleic acids by using “capture” ssDNA sequences attached to carbon nanotubes. Target SARS-CoV-2 viral RNA can be captured by ssDNA-nanotube constructs via hybridization and separated from the liquid phase in a single-tube system with minimal chemical reagents, for downstream quantitative reverse transcription polymerase chain reaction (RT-qPCR) detection. This nanotube-based extraction method enables 100% extraction yield of target SARS-CoV-2 RNA from phosphate-buffered saline in comparison to ∼20% extraction yield when using a commercial silica-column kit. Notably, carbon nanotubes enable extraction of nucleic acids directly from 50% human saliva with a similar efficiency as achieved with commercial DNA/RNA extraction kits, thereby bypassing the need for further biofluid purification and avoiding the use of commercial extraction kits. Carbon nanotube-based extraction of viral nucleic acids facilitates high-yield and high-sensitivity identification of viral nucleic acids such as the SARS-CoV-2 viral genome with a reduced reliance on reagents affected by supply chain obstacles.

KEYWORDS: viral diagnostics, qPCR, DNA/RNA extraction, carbon nanotubes, nanotechnology, nanosensors

논문정보

형식Research article
게재일2021년 06월 (BRIC 등록일 2021-06-16)
연구진국내+국외 연구진
분야 바이오·의료융합 > 바이오센싱 및 나노바이오물질

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