한빛사논문
- 조회478
KAIST
Minseok Leea, Yesol Kima, Seung Jung Yua, Sun Young Leeb, Jin Gyeng Sonb, Tae Geol Leeb, Youngbin Chod, Jennifer H. Shind, Eunjung Leea,* and Sung Gap Ima,c,*
aDepartment of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology, Daejeon 34141, Republic of Korea
bBioimaging Team, Safety Measurement Institute, Korea Research Institute of Standards and Science (KRISS), Daejeon 34141, Republic of Korea
cKAIST Institue for the NanoCentury (KINC), Korea Advanced Institute of Science and Technology, Daejeon 34141, Republic of Korea
dDepartment of Mechanical Engineering, Korea Advanced Institute of Science and Technology, Daejeon 34141, Republic of Korea
M.L. and Y.K. contributed equally to this work.
*To whom correspondence should be addressed.
Abstract
Three-dimensional in vitro cancer models have emerged as a promising tool for various cancer-related applications. However, the limited availability of the in vitro model capable of adequately recapitulating the active interactions between the cancer cells and the surrounding tumor microenvironment (TME) hampers their use for therapeutic applications. Here, it is demonstrated that the proteins adsorbed on the culture substrate significantly influence the characteristics of the cancer cells, thereby suggesting that the modulation of cell–protein interaction can be a powerful tool to construct an advanced cancer model. A series of polymers are prepared for the precise control of the surface hydrophobicity of the culture plate. Cancer cells cultured on the polymers exhibit distinct morphological transitions ranging from monolayer to spheroids with entirely different characteristics depending on the surface hydrophobicity. The poly (cyclohexyl methacrylate) surface of the highest hydrophobicity tested in this study strongly attracts albumin from the media for enhanced adsorption and induces conformational changes in albumin upon binding, leading to the formation of spheroid with the most enriched tumorigenic properties. It is believed that this finding can provide new insights when selecting the experimental strategy to appropriately mimic the complex interplay between the cancer cells and the TME.
Keywords : adsorbed proteins, albumins, cancer spheroids, copolymer films, surface hydrophobicity, tumor microenvironment
논문정보
- Research article
- 2021년 05월 (BRIC 등록일 2021-06-14)
- 국내 연구진
- 바이오·의료융합 > 바이오소재
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