한빛사논문 | BRIC

한빛사 논문

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고려대학교

Mol. Plant., Available online 5 May 2021 | https://doi.org/10.1016/j.molp.2021.05.006 Chemical control of receptor kinase signaling by rapamycin-induced dimerization

Sara Kim^1,†, Jeonghyang Park^1,†, Byeong Wook Jeon^2,3,†, Geonhee Hwang¹, Na Young Kang², Yeim We¹, Won-Young Park¹, Eunkyoo Oh^1,*, Jungmook Kim^2,3,4,*

¹Department of Life Sciences, Korea University, Seoul, Korea
²Department of Bioenergy Science and Technology, Chonnam National University, Gwangju, Korea
³Kumho Life Science Laboratory, Chonnam National University, Gwangju, Korea
⁴Department of Integrative Food, Bioscience and Biotechnology, Chonnam National University, Gwangju, Korea

^†These authors contributed equally to this work.

^*Corresponding author

Abstract

Membrane-localized leucine-rich repeat receptor kinases (LRR-RKs) sense diverse extracellular signals, and coordinate and specify cellular functions in plants. However, functional understanding and identification of the cellular signaling of most LRR-RKs remain a major challenge owing to their genetic redundancy, lack of ligand information, and subtle phenotypes of LRR-RK overexpression. Here, we report an engineered rapamycin-inducible dimerization (RiD) receptor system that triggers a receptor-specific LRR-RK signaling independent of their cognate ligands or endogenous receptors. Using the RiD-receptors, we demonstrated that the rapamycin-mediated association of chimeric cytosolic kinase domains from the BRI1/BAK1 receptor/co-receptor, but not the BRI1/BRI1 or BAK1/BAK1 homodimer, is sufficient to activate downstream brassinosteroid signaling and physiological responses and that the engineered RiD-FLS2/BAK1 activates flagellin-22-mediated immune signaling and responses. We also identified the potential function of an unknown orphan receptor in immune signaling and revealed the differential activities of SERK co-receptors of LRR-RKs. Our results demonstrated that the RiD method can serve as a synthetic biology tool for precise temporal manipulation of LRR-RK signaling and for understanding LRR-RK biology.

논문정보

형식Research article
게재일2021년 05월 (BRIC 등록일 2021-05-06)
연구진국내 연구진
분야 생명과학 > 식물학

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