한빛사논문
- 조회7,844
Abstract
Jae-Ran Lee1, Hyewon Shin 1, Jeonghoon Choi 1, Jaewon Ko 1, Seho Kim 1, Hyun Woo Lee 1, Karam Kim 1, Seong-Hwan Rho 2, Jun Hyuck Lee 2, Hye-Eun Song 2, Soo Hyun Eom 2 and Eunjoon Kim 1
1 National Creative Research Initiative Center for Synaptogenesis and Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon, Korea
2 Department of Life Science, Kwangju Institute of Science and Technology, Gwangju, Korea
To whom correspondence should be addressed
Eunjoon Kim National Creative Research Initiative Center for Synaptogenesis and Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Kuseong-dong, Yuseong-ku, Daejeon 305-701, South Korea. Tel.: +82 42 869 2633; Fax: +82 42 869 2610.
Abstract
Motor proteins not actively involved in transporting cargoes should remain inactive at sites of cargo loading to save energy and remain available for loading. KIF1A/Unc104 is a monomeric kinesin known to dimerize into a processive motor at high protein concentrations. However, the molecular mechanisms underlying monomer stabilization and monomer-to-dimer transition are not well understood. Here, we report an intramolecular interaction in KIF1A between the forkhead-associated (FHA) domain and a coiled-coil domain (CC2) immediately following the FHA domain. Disrupting this interaction by point mutations in the FHA or CC2 domains leads to a dramatic accumulation of KIF1A in the periphery of living cultured neurons and an enhancement of the microtubule (MT) binding and self-multimerization of KIF1A. In addition, point mutations causing rigidity in the predicted flexible hinge disrupt the intramolecular FHA-CC2 interaction and increase MT binding and peripheral accumulation of KIF1A. These results suggest that the intramolecular FHA-CC2 interaction negatively regulates KIF1A activity by inhibiting MT binding and dimerization of KIF1A, and point to a novel role of the FHA domain in the regulation of kinesin motors.
Keywords: coiled coil, FHA, kinesin, KIF1A, microtubule binding
논문정보
- Research article
- 2004년 03월 (BRIC 등록일 )
- 국내 연구진
- 생명과학 > 생리학
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