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구.농수식품

조회771

을지대학교 의과대학

Vet. Microbiol., Available online 23 March 2021, 109048 Intradermal co-inoculation of codon pair deoptimization (CPD)-attenuated chimeric porcine reproductive and respiratory syndrome virus (PRRSV) with Toll like receptor (TLR) agonists enhanced the protective effects in pigs against heterologous challenge

Changhoon Park^a, Myung-Shin Lee^a, Jong Hyuk Baek^b, Sun Hee Cho^b, Bang-Hun Hyun^c, Su-Hwa You^c, Sang-Ho Cha^c,*

^a Department of Microbiology and Immunology, Eulji University School of Medicine, Yongdu-dong, Jung-gu, Daejeon, Republic of Korea
^b Department of Animal Vaccine Development, BioPOA, 593-26 Dongtangiheung-ro, Hwaseong-si, Gyeonggi-do, Republic of Korea
^c PRRS research Laboratory, Viral Diseases Division, Animal and Plant Quarantine Agency, Gimcheon, 39660, Republic of Korea
^* Correspondence

Abstract

The objective of this study was to assess protective efficacy of vaccination using CPD-attenuated chimeric PRRSV and Toll like receptor (TLR) agonists (HSP70 c-terminal domain and HSPX) as adjuvants through different inoculation routes. In this study, a chimeric PRRSV composed of two field isolates was synthesized and attenuated by CPD in NSP1 as described in the previous study. The infection of the CPD-attenuated chimeric PRRSV to pigs of 3 weeks-old showed no clinical signs without pathological lesions in necropsy, while it induced improved cross immunity between its parent strains. The TLR agonists were expressed in E. coli and purified to be used. In challenge experiment, pigs of 3 weeks-old were vaccinated using the CPD-attenuated chimeric virus with the prepared TLR agonists through intramuscular or intradermal route, following heterologous challenge after 4 weeks of vaccination. In results, intramuscular or intradermal inoculation of the CPD-attenuated chimeric virus demonstrated excellent protective efficacy against heterologous challenges. Importantly, intradermal inoculation with the TLR agonists enhanced protective effects as shown in the significantly increased level of PRRSV-specific IFN-γ-SCs and cytokines in sera, and the significant reduction of pathological lesion and viral load in lung. This study suggested that the intradermal inoculation of CPD-attenuated chimeric PRRSV plus TLR agonists should be more effective for protection of pigs against diverse PRRS field viruses.

논문정보

형식Research article
게재일2021년 03월 (BRIC 등록일 2021-03-31)
연구진국내 연구진
분야 의약학 > 응용의학
구분구・농수식품

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