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Austrian Academy of Sciences

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Cell Host Microbe, Available online 12 March 2021 | https://doi.org/10.1016/j.chom.2021.02.008 Signatures of antagonistic pleiotropy in a bacterial flagellin epitope

Katarzyna Parys^1,9,8, Nicholas R. Colaianni^2,3,8, Ho-Seok Lee^1,8, Ulrich Hohmann^4,10,11, Natalie Edelbacher¹, Alen Trgovcevic¹, Zuzana Blahovska¹, Duhwa Lee¹, Alexander Mechtler¹, Zsuzsanna Muhari-Portik¹, Mathias Madalinski¹, Niklas Schandry^1,9, Isaac Rodríguez-Arévalo^1,9, Claude Becker^1,9, Elisabeth Sonnleitner⁵, Arthur Korte⁶, Udo Bläsi⁵, Niko Geldner⁷, Michael Hothorn⁴, Corbin D. Jones^3,*, Jeffery L. Dangl^2,*, Youssef Belkhadir^1,12,*

¹Gregor Mendel Institute (GMI), Austrian Academy of Sciences, Vienna BioCenter (VBC), Dr. Bohr-Gasse 3, Vienna, Austria
²Department of Biology and Howard Hughes Medical Institute, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA
³Department of Biology, Curriculum in Bioinformatics and Computational Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA
⁴Structural Plant Biology Laboratory, Department of Botany and Plant Biology, University of Geneva, 1211 Geneva, Switzerland
⁵Department of Microbiology, Immunobiology and Genetics, Max Perutz Laboratories, Center of Molecular Biology, University of Vienna, Vienna Biocenter (VBC), Dr. Bohr Gasse 9, Vienna, Austria
⁶Center for Computational and Theoretical Biology, University of Würzburg, Würzburg, Germany
⁷Department of Plant Molecular Biology, University of Lausanne, Lausanne, Switzerland
⁸These authors contributed equally
⁹Present address: Faculty of Biology, Genetics, University of Munich (LMU), 82152 Martinsried, Germany
¹⁰Present address: IMBA - Institute of Molecular Biotechnology (IMBA), Vienna Biocenter (VBC), Dr Bohr Gasse 3, Vienna, Austria
¹¹Present address: IMP - Institute of Molecular Pathology, Vienna Biocenter (VBC), Campus-Vienna, Biocenter 1, 1030 Vienna, Austria
¹²Lead contact

^*Corresponding author

Abstract

Immune systems respond to “non-self” molecules termed microbe-associated molecular patterns (MAMPs). Microbial genes encoding MAMPs have adaptive functions and are thus evolutionarily conserved. In the presence of a host, these genes are maladaptive and drive antagonistic pleiotropy (AP) because they promote microbe elimination by activating immune responses. The role AP plays in balancing the functionality of MAMP-coding genes against their immunogenicity is unknown. To address this, we focused on an epitope of flagellin that triggers antibacterial immunity in plants. Flagellin is conserved because it enables motility. Here, we decode the immunogenic and motility profiles of this flagellin epitope and determine the spectrum of amino acid mutations that drives AP. We discover two synthetic mutational tracks that undermine the detection activities of a plant flagellin receptor. These tracks generate epitopes with either antagonist or weaker agonist activities. Finally, we find signatures of these tracks layered atop each other in natural Pseudomonads.

Keywords : antagonistic pleiotropy, evolutionary constraints, directed evolution, flagellin, Pseudomonas motility, Arabidopsis thaliana immune system, ligand-receptor interaction, receptor antagonism

논문정보

형식Research article
게재일2021년 03월 (BRIC 등록일 2021-03-22)
연구진국외 연구진
분야 생명과학 > 식물학

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