차채녕 (UNIST)

한빛사논문

조회1,167

UNIST

CV File 311 kb

Biomaterials, Available online 22 January 2021, 120688 | https://doi.org/10.1016/j.biomaterials.2021.120688 Synergistic control of mechanics and microarchitecture of 3D bioactive hydrogel platform to promote the regenerative potential of engineered hepatic tissue

Suntae Kim^a,1, Myung Rae Park^b,1, Cholong Choi^a, Jeong Beom Kim^b,*, Chaenyung Cha^a,*

^aCenter for Multidimensional Programmable Matter, Department of Materials Science and Engineering, Ulsan National Institute of Science and Technology (UNIST), Ulsan, 44919, Republic of Korea
^bHans Schöler Stem Cell Research Center, Department of Biomedical Engineering, Ulsan National Institute of Science and Technology (UNIST), Ulsan, 44919, Republic of Korea

¹These authors contributed equally

^*Corresponding author

Abstract

Culturing autologous cells with therapeutic potential derived from a patient within a bioactive scaffold to induce functioning tissue formation is considered the ideal methodology towards realizing patient-specific regenerative medicine. Hydrogels are often employed as the scaffold material for this purpose mainly for their tunable mechanical and diffusional properties as well as presenting cell-responsive moieties. Herein, a two-fold strategy was employed to control the physicomechanical properties and microarchitecture of hydrogels to maximize the efficacy of engineered hepatic tissues. First, a hydrophilic polymeric crosslinker with a tunable degree of reactive functional groups was employed to control the mechanical properties in a wide range while minimizing the change in diffusional properties. Second, photolithography technique was utilized to introduce microchannels into hydrogels to overcome the critical diffusional limit of bulk hydrogels. Encapsulating hepatic progenitor cells derived via direct reprogramming of tissue-harvested fibroblasts, the application of this strategy to control the mechanics, diffusion, and architecture of hydrogels in a combinatorial manner could allow the optimization of their hepatic functions. The regenerative capacity of this engineered hepatic tissue was further demonstrated using an in vivo acute liver injury model.

Keywords : 3D hydrogel, mechanics, microarchitecture, direct reprogramming, induced hepatic progenitor cells

논문정보

형식Research article
게재일2021년 01월 (BRIC 등록일 2021-01-28)
연구진국내 연구진
분야

댓글 작성 로그인

CV 열람하기

연락처 보기