장지용 (University of Pittsburgh)

한빛사논문

조회1,037

University of Pittsburgh

CV File 153 kb

Proc. Natl. Acad. Sci. USA, January 19, 2021, 118 (3): e2005134118 | https://doi.org/10.1073/pnas.2005134118 The secretome mouse provides a genetic platform to delineate tissue-specific in vivo secretion

Jie Liu^a,b,1, Ji Yong Jang^a,1, Mehdi Pirooznia^c, Shihui Liu^a,d, and Toren Finkel^a,b,2

^aAging Institute, University of Pittsburgh School of Medicine/UPMC, Pittsburgh, PA 15219; ^bDepartment of Medicine, Division of Cardiology, University of Pittsburgh/UPMC, Pittsburgh, PA 15219; ^cBioinformatics and Computational Core Facility, National Heart, Lung, and Blood Institute, NIH, Bethesda, MD 20892; and ^dDepartment of Medicine, Division of Infectious Disease, University of Pittsburgh/UPMC, Pittsburgh, PA 15219

¹J.L. and J.Y.J. contributed equally to this work.
²To whom correspondence may be addressed.

Abstract

At present, it remains difficult to deconvolute serum in order to identify the cell or tissue origin of a given circulating protein. Here, by exploiting the properties of proximity biotinylation, we describe a mouse model that enables the elucidation of the in vivo tissue-specific secretome. As an example, we demonstrate how we can readily identify in vivo endothelial-specific secretion as well as how this model allows for the characterization of muscle-derived serum proteins that either increase or decrease with exercise. This genetic platform should, therefore, be of wide utility in understanding normal and disease physiology and for the rational design of tissue-specific disease biomarkers.

protein secretion, biotinylation, mouse model

논문정보

형식Research article
게재일2021년 01월 (BRIC 등록일 2021-01-26)
연구진국외 연구진
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