한빛사논문
- 조회988
Yong-Joon Choi, Toshiaki Takahashi, Miki Taki, Kazuaki Sawada, Kazuhiro Takahashi*
Department of Electrical and Electronic Information Engineering, Toyohashi University of Technology, 1-1 Hibarigaoka, Tempakucho, Toyohashi, Aichi, 441-8580, Japan
*Corresponding author.
Abstract
We demonstrated an optical interferometer-based surface-stress immunosensor using freestanding polymethyl methacrylate (PMMA)/parylene-C nanosheet with high sensitivity for detection of biomolecules. PMMA/parylene-C nanosheets were transferred onto a silicon substrate with microcavities to fabricate freestanding submicron-thick membrane with a sealed cavity structure. The adhesive force between the transferred parylene-C and binder parylene-C layer was measured to be 1.06–2.4 N/10 mm by tape test. Evading Debye shielding, these nanomechanical sensors allow detection of the adsorption on the membrane surface through changes in surface stress transduced by the electric charge. We optimized the density of receptors and mode of immobilization for high sensitivity. To evaluate the selectivity of the sensor, membrane deflections induced by various proteins were measured and the spectral shifts showed high selectivity only for the target antigen. The minimum limit of detection (LOD) of the sensor for human serum albumin antigen was 0.1–1 fg/mL (1.5–15 aM), which was 20,000 times lower than that of the conventional micro-cantilever sensor.
Keywords : Biosensing; Immunosensor; Fabry-perot interferometric transducer; Surface stress sensing; Nanomechanical sensor; Nanosheet transfer
논문정보
- Research article
- 2020년 10월 (BRIC 등록일 2020-11-10)
- 국외 연구진
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