박창균 (한국기초과학지원연구원, 한국화학연구원 , UST) | 한빛사논문 > 한빛사

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한국기초과학지원연구원, 한국화학연구원 , UST

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ACS Nano, Publication Date: April 15, 2020, 14(4):5135-5142 | https://doi.org/10.1021/acsnano.0c02823 Rapid Detection of COVID-19 Causative Virus (SARS-CoV-2) in Human Nasopharyngeal Swab Specimens Using Field-Effect Transistor-Based Biosensor

Giwan Seo^1,2, Geonhee Lee³, Mi Jeong Kim^1,2, Seung-Hwa Baek^2,4, Minsuk Choi², Keun Bon Ku², Chang-Seop Lee^5,6, Sangmi Jun^2,7, Daeui Park^2,4, Hong Gi Kim², Seong-Jun Kim², Jeong-O Lee³, Bum Tae Kim², Edmond Changkyun Park^1,2,8,*, Seung Il Kim^1,2,8,*

¹Research Center for Bioconvergence Analysis, Korea Basic Science Institute, Cheongju 28119, Republic of Korea
²Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114, Republic of Korea
³Advanced Materials Division, Korea Research Institute of Chemical Technology, Daejeon 34114, Republic of Korea
⁴Department of Predictive Toxicology, Korea Institute of Toxicology, Daejeon 34114, Republic of Korea
⁵Department of Internal Medicine, Jeonbuk National University Medical School, Jeonju 54986, Republic of Korea
⁶Biomedical Research Institute of Jeonbuk National University Hospital, Jeonju 54907, Republic of Korea
⁷Center for Research Equipment, Korea Basic Science Institute, Cheongju 28119, Republic of Korea
⁸Department of Bio-Analysis Science, University of Science & Technology (UST), Daejeon 34113, Republic of Korea

^*Corresponding author

Abstract

Coronavirus disease 2019 (COVID-19) is a newly emerging human infectious disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2, previously called 2019-nCoV). Based on the rapid increase in the rate of human infection, the World Health Organization (WHO) has classified the COVID-19 outbreak as a pandemic. Because no specific drugs or vaccines for COVID-19 are yet available, early diagnosis and management are crucial for containing the outbreak. Here, we report a field-effect transistor (FET)-based biosensing device for detecting SARS-CoV-2 in clinical samples. The sensor was produced by coating graphene sheets of the FET with a specific antibody against SARS-CoV-2 spike protein. The performance of the sensor was determined using antigen protein, cultured virus, and nasopharyngeal swab specimens from COVID-19 patients. Our FET device could detect the SARS-CoV-2 spike protein at concentrations of 1 fg/mL in phosphate-buffered saline and 100 fg/mL clinical transport medium. In addition, the FET sensor successfully detected SARS-CoV-2 in culture medium (limit of detection [LOD]: 1.6 × 101 pfu/mL) and clinical samples (LOD: 2.42 × 102 copies/mL). Thus, we have successfully fabricated a promising FET biosensor for SARS-CoV-2; our device is a highly sensitive immunological diagnostic method for COVID-19 that requires no sample pretreatment or labeling.

KEYWORDS:FET, biosensor, COVID-19, SARS-CoV-2, 2019-nCoV

논문정보

형식Research article
게재일2020년 04월 (BRIC 등록일 2020-11-27)
연구진국내 연구진
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