조윤경 (기초과학연구원, UNIST) | 한빛사논문 > 한빛사

한빛사논문

조회754

기초과학연구원, UNIST

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Biomaterials, Available online 23 September 2020, 120420 | https://doi.org/10.1016/j.biomaterials.2020.120420 Adaptive architecture and mechanoresponse of epithelial cells on a torus

Sun-Min Yu^a,b, Bo Li^a, François Amblard^a,b, Steve Granick^a,c, Yoon-Kyoung Cho^a,b,*

^aCenter for Soft and Living Matter, Institute for Basic Science (IBS), Ulsan, 44919, Republic of Korea
^bDepartment of Biomedical Engineering, Ulsan National Institute of Science and Technology (UNIST), Ulsan, 44919, Republic of Korea
^cDepartment of Chemistry, Ulsan National Institute of Science and Technology (UNIST), Ulsan, 44919, Republic of Korea

^*Corresponding author

Abstract

Curvature is a geometric feature widely observed in the epithelia and critical to the performance of fundamental biological functions. Understanding curvature-related biophysical phenomena remains challenging partly owing to the difficulty of quantitatively tuning and measuring curvatures of interfacing individual cells. In this study, we prepared confluent wild-type Madin–Darby canine kidney cells on a torus structure presenting positive, zero, and negative Gaussian curvatures with a tubule diameter of 2–7 cells and quantified the mechanobiological characteristics of individual cells. Cells on the torus surface exhibited topological sensing ability both as an individual cell and collective cell organization. Both cell bodies and nuclei, adapted on the torus, exhibited local Gaussian curvature-dependent preferential orientation. The cells on the torus demonstrated significant adjustment in the nuclear area and exhibited asymmetric nuclear position depending on the local Gaussian curvature. Moreover, cells on top of the torus, where local Gaussian curvature is near zero, exhibited more sensitive morphological adaptations than the nuclei depending on the Gaussian curvature gradient. Furthermore, the spatial heterogeneity of intermediate filament proteins related to mechanoresponsive expression of the cell body and nucleus, vimentin, keratin and lamin A, revealed local Gaussian curvature as a key factor of cellular adaptation on curved surfaces.

Keywords : Gaussian curvature; Mechanobiology; Cell morphology; Nucleus; Vimentin; Lamin A

논문정보

형식Research article
게재일2020년 09월 (BRIC 등록일 2020-10-05)
연구진국내 연구진
분야 바이오·의료융합 > 바이오센싱 및 나노바이오물질

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